Boston University

Centre National De La Recherche Scientifique

L'institut Polytechnique De Bordeaux

L'universitÉ De Bordeaux

Catherine Klapperich

Mark W Grinstaff

Jacqueline Callihan Linnes

James Galagan

Uros Kuzmanovic

Andy Fan

Mingfu Chen

Thuy Nguyen

Margaret Chern

R Baer

Chloe Grazon

Natalia M Rodriguez

George Woodman Pratt, Iv

Allison Dennis

Marjon Zamani

Described herein are methods, compositions and processes related to a microbial-based biosensor system for the detection of small molecules and analytes based on an analyte-responsive transcription factor-DNA binding mechanism with either a ratiometric fluorescent output through F&#xf6;rster resonance energy transfer (FRET) or a redox sensor output for the quantification of the target analyte with high sensitivity.

Microbial-based biosensors

The invention features a fully-integrated rapid molecular diagnostic device that is low-cost, easy to manufacture, and simple to use. The device can serve as a molecular diagnostic platform for any disease, requiring little or no preparation or customization and can be made from simple materials (e.g., paper and adhesive film), making it inexpensive, portable, and disposable. The invention also provides methods of using the device for detection of one or more targets in a sample.

Detection device having capture region and detection region

Disclosed herein are antibodies capable of specifically binding to tenofovir (TFV), a key small molecule drug for both the treatment and prevention of HIV, and a competitive lateral flow assay that uses these antibodies to monitor urine samples for the presence of the drug. The assay can be deployed as a point-of-care device for adherence monitoring.

Tenofovir detection assay

Growing community of inventors

Brookline, MA, United States of America

Catherine Klapperich