University of Nottingham

Merck Patent Gmbh

Amersham International Plc

Catherine E Rees

Gordon Sydney Stewart

Stephen Paul Denyer

Sabah Abdel Jassim

Katalin Rostas-Mulligan

Simon Fearon Park

Paul Williams

Barrie Walsham Bycroft

Benjamin Swift

Philip John Hill

Paul Stead

Michael K Winson

Nigel J Bainton

Siri R Chhabra

A method for testing for target Mycobacteria in a reaction mixture comprising the steps of: providing a reaction mixture; admixing a bacteriophage with the reaction mixture under conditions suitable to allow the bacteriophage to infect any target Mycobacteria present in the reaction mixture; allowing time for the bacteriophage to lyse infected live target Mycobacteria; and analysing in said reaction mixture DNA from the lysed Mycobacteria to identify a signature DNA sequence that occurs in the target Mycobacteriumspedes.

Mycobacteria detection using bacteriophages

A method for testing for target bacteria involves adding bacteriophage to a sample to infect the bacteria in the sample; killing extracellular bacteriophage without killing phage-infected bacteria; amplifying bacteriophage remaining in the sample; and causing the bacteriophage to infect reporter bacteria and thereby produce an observable signal. The reporter bacteria are genetically engineered to have an indicator gene which on expression gives rise to a detectable signal, wherein expression of the indicator gene is initiated on bacteriophage infection of the bacteria.

Genetically engineered reporter bacteria for the detection of

Autoinducer

Methods for rapid microbial detection

Growing community of inventors

Nottingham, United Kingdom

Catherine E Rees