Danisco US Inc.

Dupont Nutrition Biosciences Aps

Casper Willem Vroemen

Richard Ray Bott

David Aaron Estell

Clement K Choy

Andrew Shaw

Scott Douglas Power

Kathleen A Clarkson

Martijn Scheffers

Wolfgang Aehle

Bo Zhang

Walter Weyler

Thomas P Graycar

Luis Gustavo Cascao-Pereira

Sandra W Ramer

Melodie Estabrook

Pieter Van Solingen

Ling Hua

Jing Ge

Viktor Alekseyev

Paula Johanna Maria Teunissen

George R England

Claudine Chang

Peter Edvard Degn

Jacquelyn A Huitink

Daniel Esteban Torres Pazmino

Mansi Goyal

Elin Petersen

Chris Leeflang

Marco Van Brussel

Zhengong Zhang

Marc Kolkman

Zhongmei Tang

Igor Nikolaev

Victoria Huang

James T Kellis

Brian E Jones

Viktor Yuryevich Alekseyev

Karsten M Kragh

Piet Van Solingen

Martijin Scheffers

Martijn Silvan Scheffers

David L Wong

Su Yin Maria Chow

Scheffers Martijn

Piet Van Sollingen

Marco Van Brussel-Zwijnen

Aspects of the present disclosure include glucoamylase (GA) variants having at least one improved property over a parent GA, compositions containing the GA variants, nucleic acids encoding the GA variants, and methods for producing and using the same. In some aspects, the GA variant is a variant of a parent GA from

Glucoamylase variants

A fungal alpha-amylase is provided from(AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

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The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

Variants of glucoamylase

The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

Glucoamylase variants with altered properties

Growing community of inventors

Palo Alto, CA, United States of America

Casper Willem Vroemen