Cellular Dynamics International, Inc.

Fujifilm Cellular Dynamics, Inc.

Casey Stankewicz

Wen Bo Wang

Steven James Kattman

Peter Fuhrken

Nathan Meyer

Nathaniel Beardsley

Matt Riley

Lucas Chase

Matthew George

Deepika Rajesh

Steven Kattman

Provided herein are methods of enriching a retinal pigment epithelium (RPE) cell population derived from stem cells. Such a method may comprise removing contaminating cells through the depletion of CD24 positive cells, CD56 positive cells, and/or CD90 positive cells from a starting population of RPE cells.

Macs-based purification of stem cell-derived retinal pigment epithelium

MACS-based purification of stem cell-derived retinal pigment epithelium

Methods and composition for the production of cardiomyocytes from differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including differentiating pluripotent stem cells in a large volume of suspension culture in the presence of ROCK inhibitors are described. In further aspects, methods for differentiation of stem cells into cardiomyocytes that overcome variability between different stem cell clones and different batch of culture medium are provided.

Cardiomyocyte production

Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including priming stem cells for neural differentiation in a culture medium essentially free of growth factors such as FGF and TGF&#x3b2;. As an advantage, the neural cells may be provided with improved consistency and purity.

Priming of pluripotent stem cells for neural differentiation

Methods and composition for differentiation of pluripotent stem cells are provided. For example, in certain aspects methods including screening of optimal differentiation conditions for a selected stem cell clone in a selected batch of culture medium and use of the determined optimal condition for differentiation into a specific cell lineage.

Titration of differentiation medium components

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Madison, WI, United States of America

Casey Stankewicz