Novo Nordisk A/s

Carsten Mailand Hjort

Sven Hastrup

Fred Hagen

Helle Fabricius Woldike

Torben Halkier

Lene Venke Kofod

Inge Helmer Knap

Martin Schulein

Shamkant Anant Patkar

Grethe Norskov Rasmussen

Jan Mo Mikkelsen

The present invention relates to cellulase preparations consisting essentially of a homogeneous endoglucanase component. The cellulase preparation may be employed in the treatment of cellulose-containing fabrics for harshness reduction, for color clarification, or to provide a localized variation in the color of such fabrics, or in the treatment of paper pulp.

Cellulase preparation comprising an endoglucanase enzyme

An isolated polypeptide having &agr;-galactosidase activity and characterized as having a pH optimum in the range of 5.0-7.0, and a temperature optimum within the range of 50-70&deg; C. The &agr;-galactosidase is derived from

&agr;-galactosidase enzyme

A cellulase preparation consisting essentially of a homogeneous endoglucanase component which is immunoreactive with an antibody raised against a highly purified .about.43 kD endoglucanase derived from Humicola insolens, DSM 1800, or which is homogeneous to said .about.43 kD endoglucanase, may be employed in the treatment cellulose-containing fabrics for harshness reduction or color clarification or to provide a localized variation in the color of such fabrics, or it may be employed in the treatment of paper pulp.

.alpha.-galactosidase enzyme

Fungal protein disulfide isomerase

The present invention relates to isolated and purified cellulose- or hemicellulose-degrading enzymes, comprising a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, wherein the carbohydrate binding domain has the following core sequence: ##STR1## wherein the enzyme is endogenous to a strain of a fungus other than Trichoderma or Phanerochaete.

Enzyme capable of degrading cellulose or hemicellulose

The present invention relates to cellulose- or hemicellulose-degrading enzymes which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain comprises the following amino acid sequence. ##STR1## or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.

A cellulose- or hemicellulose-degrading enzyme which is derivable from a fungus other than Trichoderma or Phanerochaete, and which comprises a carbohydrate binding domain homologous to a terminal A region of Trichoderma reesei cellulases, which carbohydrate binding domain amino acid sequence (.alpha.) or a subsequence thereof capable of effecting binding of the enzyme to an insoluble cellulosic or hemicellulosic substrate.

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Roskilde, Denmark

Carsten Mailand Hjort