Novozymes A/s

Novo Zymes North America, Inc

Novozyms A/s

Carsten Mailand Hjort

Henrik Pedersen

Carsten Schau Andersen

Sven Pedersen

Tove Christensen

Anders Vikso-Nielsen

Tomoko Matsui

Shinobu Takagi

Shiro Fukuyama

Peter Jan Punt

Hiroaki Udagawa

Eric Allain

Rikako Taira

Frank H J Schuren

Shamkant Anant Patkar

Soren Ebdrup

Jens Nielsen

Peter Rahbek Ostergaard

Anders Viksoe-Nielsen

Anders Vikso Nielsen

Heinz-Josef Deussen

Magali Zundel

Soren Christensen

Cees A M J J Van Den Hondel

C A M J J Van Den Hondel

P J Punt

F H J Schuren

Cees Van Den Hondel

The present disclosure relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The disclosure also relates to nucleic acid constructs, vectors and host cells including the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.

Fungal transcriptional activator useful in methods for producing polypeptides

The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.

Enzymes for starch processing

The present invention relates to a process for enzymatic hydrolysis of granular starch into a soluble starch hydrolysate at a temperature below the initial gelatinization temperature of said granular starch.

Starch process

The present invention relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.

Process of producing a starch hydrolysate

An isolated mutant host cell produced by disrupting or deleting, in a parent cell, a nucleic acid sequence encoding an oxaloacetate hydrolase, or a promoter thereof, which results in the mutant cell producing less of the oxaloacetate hydrolase than the parent cell is disclosed. The disclosure also relates to recombinant methods for producing the products such as polypeptides.

Oxaloacetate hydrolase deficient fungal host cells

The present invention relates to isolated nucleic acid sequences encoding polypeptides having oxaloacetate hydrolase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.

Methods for producing citric acid using host cells deficient in oxaloacetate hydrolase

An esterase isolated fromis capable of stereoselective hydrolysis of chiral esters and also has arylesterase activity (EC 3.1.1.2) and feruloyl esterase activity (EC 3.1.1.73). The esterase has only a limited homology to known amino acid sequences.

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The present invention relates to methods of producing a polypeptide comprising culturing a mutant of a parent cell, wherein the mutant produces less oxaloacetate hydrolase than the parent cell, wherein the oxaloacetate hydrolase (i) has an amino acid sequence that has at least% identity with amino acids 1-341 of SEQ ID NO: 2: (ii) is encoded by a nucleic acid sequence having at least 90% homology with a nucleotide sequence comprising nuclotides 1157-1411, 1504-1651 and 1764-2383 of SEQ ID NO: 1: (iii) is encoded by a nucleic acid sequence which hybridizes under high stringency conditions with the nucleic acid sequence of SEQ ID NO: 1, the cDNA sequence of SEQ ID NO: 1, the complementary strand of SEQ ID NO: 1, and/or the complementary strand of the cDNA sequence of SEQ ID NO: 1: and/or (iv) a subsequence of one or both of (i) and (ii), wherein the subsequence encodes a fragment that has oxaloacetate hydrolase activity.

Method of producing a polypeptide using oxaloacetate hydrolase deficient fungal host cells

The present invention relates to protein disulfide isomerases which are encoded by a nucleic acid sequence which hybridizes with (i) the DNA sequence of SEQ ID NO:1 or (ii) the DNA sequence of SEQ ID NO:2, under the following conditions: presoaking in 5&times;SSC and prehybridizing for 1 h at &tilde;40&deg; C. in a solution of 5&times;SSC, 5&times;Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 &mgr;g of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 &mgr;Ci 32-P-dCTP labelled probe for 18 h at &tilde;40&deg; C. followed by washing three times in 2&times;SSC, 0.2% SDS at 40&deg; C. for 30 minutes; and fragments thereof. The present invention also relates to DNA sequences encoding the protein disulfide isomerases, compositions comprising said protein disulfide isomerases and methods of use thereof.

Fungal protein disulfide isomerase

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Smorum, Denmark

Carsten Mailand Hjort