The University of North Carolina at Chapel Hill

Molecular Devices Corporation

Xanthon, Inc.

Carson R Loomis

H Holden Thorp

Mary E Napier

Jinheung Kim

Dean H Johnston

Mark F Sistare

Allen E Eckhardt

Robert H Oakley

Shuntai Wang

Described herein are methods of identifying a transmembrane receptor (TMR) agonist and compounds identified by this method. The TMR agonist (TMRA) is capable of activating TMR signaling while exhibiting reduced TMR internalization over a control compound.

Methods of identifying reduced internalization transmembrane receptor agonists

A method of detecting a nucleic acid (e.g., DNA, RNA) that contains at least one preselected base (e.g., adenine, guanine, 6-mercaptoguanine, 8-oxo-guanine, and 8-oxo-adenine) comprises (a) reacting the nucleic acid with a transition metal complex capable of oxidizing the preselected base in an oxidation-reduction reaction; (b) detecting the oxidation-reduction reaction; and (c) determining the presence or absence of the nucleic acid from the detected oxidation-reduction reaction at the preselected base. The method may be used in a variety of applications, including DNA sequencing, diagnostic assays, and quantitative analysis.

Microelectronic device for electrochemical detection of nucleic acid hybridization

Electrochemical detection of nucleic acid hybridization

Microelectronic device

A polymer-electrode including (a) a substrate having a conductive working surface; and (b) a polymer layer on the conductive working surface. The polymer layer has a plurality of microfluidic reaction openings distributed throughout the layer. An oligonucleotide probe can be attached to the polymer layer and is available to capture target nucleic acid. A soluble mediator can diffuse freely and transfer electrons from the preselected base in the hybridized nucleic acid to the conductive working surface of the substrate. An electronic signal generated from the electron transfer reaction is detected and quantitated.

Polymer-electrodes for detecting nucleic acid hybridization and method

Growing community of inventors

Durham, NC, United States of America

Carson R Loomis