Advanced Liquid Logic, Inc.

Carrie A Graham

Allen E Eckhardt

Vamsee K Pamula

Ramakrishna Sista

Theodore M Winger

Tong Wang

Gajendrasinh Raolji

Raveendra Dayam

Yalin Xiong

Lisa Perkins

Ning Wu

Sanjay Saha

Aspects of embodiments may include methods for automated enzymatic detection of glucose-6-phosphate dehydrogenase (G6PD) activity. Aspects of embodiments may include methods for enzymatic detection of G6PD activity in droplets in oil. Aspects of embodiments may include a system including a droplet actuator. Aspects of embodiments may include a treatment method.

Glucose-6-phosphate dehydrogenase assays

The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.

Enzyme assays on a droplet actuator

Methods of conducting an assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity are provided. The methods include concentrating the glycoprotein having enzymatic activity by capturing the glycoproteins from the input sample on a solid support, washing the glycoproteins captured on a solid support to remove unbound portions of the input sample, adding an enzyme substrate to the output sample comprising glycoproteins having enzymatic activity, and measuring enzymatic modification of the substrate. In some examples, the capturing of the glycoprotein and subsequent washing steps are performed in one or more droplets in oil.

Assay for measuring enzymatic modification of a substrate by a glycoprotein having enzymatic activity

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Raleigh, NC, United States of America

Carrie A Graham