University of California

Leland Stanford Junior University

Los Alamos National Security, LLC

Venn Biosciences Corporation

Columbia University

Children's Medical Center Corporation

The United States Government As Represented by the Department of Veterans Affairs

Brookhaven Science Associates, Lcc

Ganna Bio, Inc.

Carolyn Ruth Bertozzi

Ellen May Sletten

Peng Wu

Jennifer A Prescher

Jeremy Michael Baskin

Brian L Carlson

Isaac S Carrico

Eliana Saxon

Nicholas J Agard

Peyton Shieh

Chelsea Gloria Gordon

John C Jewett

Peter Robinson

Cheng-Ting Tsai

Spencer J Williams

Joseph D Mougous

Ramesh Jasti

Christina Woo

Mary Sloan Siegrist Palmore

Elena A Dubikovskaya

Richard A Mathies

Christopher J Chang

Steven D Rosen

Carlito Bangeles Lebrilla

Jie Song

Jon O Nagy

Erik Douglas

Andrew M Bradbury

Matthew B Francis

Amy Twite

Hiroaki Onoe

Han Xiao

Elliot C Woods

Genevieve C Van De Bittner

Lisa A Marcaurelle

Elena C Rodriguez

Melissa Gray

Shih-Chia Hsiao

Aldo Mario Eduardo Silva Carrascoso

Ravi Chandra

Nicholas Toriello

Lieza Marie Araullo Danan-Leon

John Kehoe

Alexander K Zettl

Ronald N Zuckermann

Benjamin J Smith

Anton Wyss-Coray

Wayne R Spevak

Brian Goodman

Brian Phillip Smart

Ravindranath Mukkamala

Kevin J Yarema

Dawn E Verdugo

Mark D Bednarski

Oleg Gang

Antoni P Tomsia

Richard D Cummings

Seung-Wuk Lee

Mark Alan Breidenbach

Mindy Tsai

Stephen J Galli

Lara K Mahal

Hopin Hu

Namita Bisaria

Falguni Dasgupta

Eduardo Saiz

Andreas Stahl

Dominique Baude

Michael C Bassik

Amy Helene Fluitt

Cheng-ting Tsai

Allison Cohen

Kaori Mukai

Qing Chen

Shih-Ting Wang

Ciaran Lawlor

Ryan A Flynn

Mohui Wei

Steven Banik

Kayvon Pedram

Green Ahn

John Vincent Pluvinage

Paresh Agarwal

Michael Haney

Austin A Pitcher

Mireille Kamariza

Krishnan K Palaniappan

Jason S Rush

Frederick Tomlin

Xing Chen

Ulla Gerling-Driessen

Provided are conjugates including a targeting moiety that binds to a cell surface molecule of a target cell and a target cell surface-editing enzyme. Also provided are compositions and kits that include the conjugates, as well as methods of using the conjugates. Methods of making conjugates are also provided.

Conjugates for targeted cell surface editing

Methods are provided for detecting antigen binding agents in samples. Aspects of the methods include detection of the aggregation of antigen binding agents with polynucleotide-bound antigens by sensitive proximity-based association of the antigen-bound polynucleotides. Aspects of the methods also include methods for the detection of such proximity-based association through nucleic acid amplification. In addition, compositions, e.g., reagents, kits, and devices, useful in practicing various embodiments of the methods are provided.

Methods for detecting agglutination and compositions for use in practicing the same

The present invention provides modified cycloalkyne compounds; and method of use of such compounds in modifying biomolecules. The present invention features a cycloaddition reaction that can be carried out under physiological conditions. In general, the invention involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating a covalently modified biomolecule. The selectivity of the reaction and its compatibility with aqueous environments provide for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).

Compositions and methods for modification of biomolecules

Provided herein are compositions, methods, kits and systems for treating cells, tissues and subjects to alter age-related biology (e.g., to study or to treat age-related diseases and conditions). In particular, provided herein are compositions, methods, and uses for inhibition or modification of sialic acid or its cognate receptor to restore phagocytosis in aged cells.

Compositions and methods for treating age-related diseases

A series of carbohydrate-dye conjugates, as well as a method for detection of pathogenic or other organisms (e.g., bacteria) using the same are provided. The carbohydrate-dye conjugate can be enzymatically incorporated into live and active (viable) bacteria of interest for facile detection of said bacteria. The conjugate incorporation is achieved by utilizing one or more of the enzymes that are endogenous to the bacteria of interest, which can incorporate the conjugate via the conjugate's carbohydrate. A detectable signal is produced by the conjugate's dye only upon incorporation into the bacteria of interest, due to the changes in the dye's local environment upon incorporation. The conjugate may be metabolically incorporated into the fatty outer membrane of a bacterial cell wall, which provides a distinctly hydrophobic environment for the conjugate's dye, causing it to produce a detectable signal.

Methods for detecting mycobacteria with solvatochromic dye conjugates

Provided are bifunctional molecules that include a first moiety that specifically binds a cell surface molecule or extracellular molecule, and a second moiety that specifically binds a lysosomal targeting molecule. The bifunctional molecules find use, e.g., for targeted degradation of cell surface and extracellular molecules (e.g., proteins) via the endosomal/lysosomal pathway. Also provided are compositions and kits that include the bifunctional molecules, as well as methods of using the bifunctional molecules. Methods of making bifunctional molecules are also provided.

Bifunctional molecules for lysosomal targeting and related compositions and methods

The present disclosure relates to glycan-modified nucleic acids, including short interfering RNA molecules. The glycan-modified nucleic acids include an oligosaccharide moiety that is bond to the nucleic acid and that contains a multiple antennary complex type N-glycan. The disclosure also relates to pharmaceutical compositions containing such glycan-modified nucleic acids, and methods for making and using such glycan-modified nucleic acids.

Glycan modified short interfering RNA

The current invention pertains compositions and methods to generate compositions providing stability to biomolecules, including providing physiologically stable and functional DNA origami-based drug/gene delivery carriers by surface coating with the oligo-ethylene glycol conjugated peptoids of Formulas (I), (II), and (III).

Side chain modified peptoids useful as structure-stabilizing coatings for biomaterials

Methods and reagents for multiplex detection of antibodies are disclosed. In particular, the invention relates to multiplex detection of antibodies using antigen-DNA and antibody-binding agent-DNA conjugates carrying DNA barcodes for identifying and quantitating disease-relevant antibody isotypes, such as those involved in allergic responses, autoimmune diseases, infections, and inflammation.

Multiplex isotype-specific antibody detection

Provided herein are methods for identifying new biomarkers for various diseases using proteomics, peptidomics, metabolics, proteoglycomics, glycomics, mass spectrometry and machine learning. The present disclosure also provides glycopeptides as biomarkers for various diseases such as cancer and autoimmune diseases.

Identification and use of glycopeptides as biomarkers for diagnosis and treatment monitoring

Methods are provided for treatment of cancer cells, in a regimen comprising contacting the cancer cells with an inhibitor on NGly1, optionally in combination with a direct proteasome inhibitor.

Agents that inhibit NGLY1 and methods of use thereof

Pharmaceutical compositions are provided for extending the serum half-life of a therapeutic agent. The composition may include a strained alkyne-labeled therapeutic agent. Also provided is a strained alkyne-carrier protein adduct including a vinyl thioether-linkage to a cysteine residue of the carrier protein. Methods of chemoselectively modifying a carrier protein are provided, that include conjugating a cysteine residue of a carrier protein with a cyclooctyne-labeled cargo agent to produce a vinyl thioether-linked conjugate. Also provided is a method of increasing the in vivo half-life of a bioactive agent including administering a strained alkyne-labeled bioactive agent to a subject.

Chemoselective modification of a carrier protein with a strained alkyne-labeled cargo agent

Growing community of inventors

Berkeley, CA, United States of America

Carolyn Ruth Bertozzi