Danisco US Inc.

The Goodyear & Tire Rubber Company

Butamax Advanced Biofuels LLC

Other

E.i. Dupont De Nemours and Company

Caroline M Peres

Joseph Charles McAuliffe

Derek H Wells

Zachary Quinn Beck

Gopal K Chotani

Marguerite A Cervin

Gregory Marshall Whited

Karl John Sanford

Andrei Miasnikov

Michael C Miller

Jeff P Pucci

Yuliya A Primak

Fernando Valle

Frank James Feher

Aaron Scott Puhala

Alex Toftgaard Nielsen

Richard La Duca

Dmitrii V Vaviline

Andrew C Eliot

Eugenio Ferrari

Richard Ray Bott

James T Kellis, Jr

Daniel P O'Keefe

Walter Weyler

Mark J Nelson

Jean-Francois Tomb

Anita Van Kimmenade

Carol Marie Fioresi

Michael Dauner

Jahnavi Chandra Prasad

Christopher Lee Rife

Rachel E Muir

Ritu Bhalla

Larry Cameron Anthony

Robert Balcarcel

Joseph C Mcauliffe

Alex T Nielsen

The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organismsDSM 20601,EG2, and).

Recombinant microorganisms for enhanced production of mevalonate, isoprene, and isoprenoids

The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.

Compositions and methods for producing isoprene

The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms via the heterologous expression of the mvaE and mvaS genes from the organismsDSM 20601,EG2, and

Production of mevalonate, isoprene, and isoprenoids using genes encoding polypeptides having thiolase, HMG-CoA synthase and HMG-CoA reductase enzymatic activities

The invention relates to the fields of industrial microbiology and alcohol production including production of yeast products with features suitable for transport, storage, and utilization in fermentation.

Method for the production of yeast

The invention provides for methods for the production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in cells via the heterologous expression of phosphoketolase enzymes.

Utilization of phosphoketolase in the production of mevalonate, isoprenoid precursors, and isoprene

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased Kfor NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

Glycerol 3-phosphate dehydrogenase for butanol production

The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.

Methods of producing isoprene and a co-product

Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A-P-M-A, where Aand Aare direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.

Method for amplifying locus in bacterial cell

Glycerol 3- phosphate dehydrogenase for butanol production

This invention relates to recombinant microorganisms capable of producing isoprene and isoprene production with the use of such recombinant microorganism with good efficiency. In this invention, functional activity of the ispA gene is altered to reduce the production of isoprenoid molecules in recombinant cells engineered to produce isoprene or in cells otherwise susceptible to isoprenoid accumulation during fermentation. This decreased ispA gene functional activity enables enhanced synthesis of isoprene in a host microorganism.

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Provided herein are improved compositions and methods for the increased production of isoprene. Also provided herein are improved compositions and methods for the increased production of heterologous polypeptides capable of biological activity.

Compositions and methods of PGL for the increased production of isoprene

Growing community of inventors

Palo Alto, CA, United States of America

Caroline M Peres