Degussa Aktiengesellschaft

Evonik Degussa Gmbh

Other

Caroline Kreutzer

Brigitte Bathe

Walter Pfefferle

Bettina Moeckel

Georg Thierbach

Thomas Hermann

Mike Farwick

Hermann Sahm

Lothar Eggeling

Achim Marx

Stephan Hans

Bettina Mockel

Miroslav Patek

Mechthild Rieping

Wilfried Claes

Silke Jerrentrup

Michael Binder

Bettina Möckel

Volker F Wendisch

Stefan Hans

Monika Martens

Mike Garwick

Doris Rittmann

Iris WIEGRÄBE

A method for producing an L-amino acid comprising: a) cultivating in a medium containing glycerol a recombinant coryneform bacteria which produces the desired L-amino acid and which expresses at least one heterologous polynucleotide of glycerol metabolism, such as glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, gipT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and/or talC, and b) isolating the desired L-amino acid. Preferably, the pathways producing the desired L-amino acid in the coryneform bacteria are amplified.

Method for the fermentative production of L-amino acids with the aid of coryneform bacteria capable of using glycerin as the only carbon source

The invention relates to mutants and alleles of the oxyR gene of coryneform bacteria coding for variants of the OxyR transcription regulator and processes for producing amino acids using bacteria which comprise these alleles.

Alleles of the oxyR gene from coryneform bacteria

An isolated mutant of a coryneform bacterium comprising a gene coding for a polypeptide having GTP-pyrophosphate kinase activity, wherein said polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids other than L-proline is present in position 38 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having GTP-pyrophosphate kinase enzyme activity, a vector comprising the isolated polynucleotide, a recombinant microorganism comprising the vector, and a process for preparing the recombinant coryneform bacterium is described. A method for over-expressing a GTP-pyrophosphate kinase, a method of preparing an L-amino acid, an L-lysine comprising and L-tryptophan comprising feed is also described.

Alleles of the rel gene from coryneform bacteria

An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.

Alleles of the PRPD1 gene from coryneform bacteria

Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.

Nucleotide sequences for encoding of the lysR2-gene

The present invention relates to polynucleotides corresponding to the luxR gene and which encode a LuxR transcriptional activator, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having LuxR transcriptional activation activity.

Nucleotide sequences coding for the LuxR gene

The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.

Coryneform bacteria which produce chemical compounds I

The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.

Nucleotide sequences which code for the ccpA2 gene

The present invention provides isolated polynucleotides containing a polynucleotide sequence which is:

Attenuated CCPA1 modified bacterial cell and its use for the preparation of L-amino acids

The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

L-Lysine-producing corynebacteria and process for the preparation of L-lysine

The invention relates to alleles of the aceA gene from coryneform bacteria coding for isocitrate lyases and processes for the fermentative production of L-lysine using bacteria that contain these alleles.

Alleles of the aceA gene from coryneform bacteria

Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

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Melle, Germany

Caroline Kreutzer