Centre National De La Recherche Scientifique

Etablissement Francais Du Sang

Universitè De Montpellier

Universite Claude Bernard – Lyon1

Université Claude Bernard Lyon I

Université De Montpellier 1

Carole Farre

Carole Chaix

Jean-Jacques Vasseur

François Morvan

Albert Meyer

Chantal Fournier-Wirth

Myriam Lereau

Jean-François Cantaloube

Julie Mayen

Francois Morvan

Jean-Francois Cantaloube

Gabriel De Crozals

The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.

Modified oligonucleotides comprising thiol functions and use thereof for detecting nucleic acids

The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

Thiol compounds and the use thereof for the synthesis of modified oligonucleotides

The invention concerns a material composed of a porous support on which functionalized nanoparticles are grafted by covalent bonding, characterized in that at least part of the nanoparticles grafted by covalent bonding is housed inside surface pores of the support, and in that the support is silica-based and is in the form of porous particles of heterogeneous shape and size, the size of the particles being larger than 1 &#x3bc;m and preferably within the range of 5 to 200 &#x3bc;m. The invention further concerns a growth method for oligonucleotides or peptides characterized in that growth is performed on a material formed of a porous support on which functionalized nanoparticles are grafted by covalent bonding, characterized in that at least a part of the nanoparticles grafted by covalent bonding is housed inside surface pores of the support.

Material for supported synthesis and method for growing oligonucleotides or peptides

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Lyons, France

Carole Farre