Biomerieux, Inc.

Carole Chaix-Bauvais

Bernard F Mandrand

Francis Garnier

Corinne Moustrou

Nicolas Spinelli

Hugues Brisset

Aude-Emmanuelle Navarro

The invention relates to bi-functionalised metallocenes of general formula (I) where Me=a transition metal, preferably chosen from Fe, Ru and Os, Y and Z, when identical are selected from &#x2014;(CH)&#x2014;O&#x2014;, (CH)&#x2014;O&#x2014;[(CH)&#x2014;O]&#x2014; and &#x2014;(CH)&#x2014;CONH&#x2014;(CH)&#x2014;O&#x2014;, or Y=&#x2014;(CH)&#x2014;NH&#x2014; and Z=&#x2014;(CH)&#x2014;COO&#x2014;,=a whole number from 3 to 6 inclusive, p=a whole number from 1 to 4 inclusive, q=a whole number from 0 to 2 inclusive, r=a whole number from 0 to 2 inclusive, s=a whole number from 2 to 5 inclusive, t=a whole number from 3 to 6 inclusive, R and R'=H atoms or are protective groups used in oligonucleotide and peptide synthesis, where at least one of R or R&#x2032; is protective group used in oligonucleotide and peptide synthesis and R and R&#x2032; are as defined below: (i) when Z and Y are selected from (CH)&#x2014;O&#x2014;, &#x2014;(CH)&#x2014;O&#x2014;[(CH)&#x2014;O]&#x2014; and &#x2014;(CH)&#x2014;CONH&#x2014;(CH)&#x2014;O&#x2014;, then R and R&#x2032; are protective groups used in oligonucleotide synthesis and R is a group which can leave a free OH group after deprotection, preferably a photolabile group such as monomethroxythoxytrityl, dimethoxytrityl, t-butyldimethylsilyl, acetyl or trifluroacetyl, and R&#x2032; is a phosphorylated group which can react with a free OH, preferably a phosphodiester, phosphoramidite or H-phosphonate and (ii) when Y=&#x2014;(CH)&#x2014;NH&#x2014; and Z=&#x2014;(CH)&#x2014;COO&#x2014;, then R is a protective group used in the synthesis of peptides and is an amino-protecting group, preferably 9-fluorenyloxycarbonyl, t-butoxycarbonyl or benzyloxycarbonyl and R&#x2032;=H. The above is applied in marking.

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Chaponnay, France

Carole Chaix-Bauvais