Centre National De La Recherche Scientifique

Etablissement Francais Du Sang

Biomerieux, Inc.

University Claude Bernard Lyon I

Universitè De Montpellier

University Claude Bernard - Lyon 1

Université De Montpellier 1

Carole Chaix

Carole Farre

Jean-Jacques Vasseur

François Morvan

Albert Meyer

Myriam Lereau

Jean-François Cantaloube

Chantal Fournier-Wirth

Julie Mayen

Bernard F Mandrand

Gabriel De Crozals

Jean-Francois Cantaloube

Francois Morvan

Alain Laurent

Daniela Zsoldos

Nicolas Spinelli

Aurelie Bouchet

The present invention relates to a modified oligonucleotide having two or more thiol functions, which can be immobilized on a gold surface or on a grafted surface, in particular a surface comprising at least one carbon-carbon double bond or carbon-carbon triple bond or haloacetamide functions, preferably maleimide or acrylamide functions. The invention also relates to a method for detecting a nucleic acid in a biological sample comprising a step of detecting hybridization between a modified oligonucleotide and a target nucleic acid amplified from the biological sample. The invention relates more particularly to a method for detecting, genotyping or sequencing a pathogenic organism, preferably a virus.

Modified oligonucleotides comprising thiol functions and use thereof for detecting nucleic acids

The present invention relates to a thiol compound suitable for forming a chain of oligomers that can be grafted to an oligonucleotide. The invention also relates to an oligonucleotide grafted by such a compound, thus having one or more thiol functions, suitable for being immobilized on a gold surface or on a grafted surface.

Thiol compounds and the use thereof for the synthesis of modified oligonucleotides

The present invention relates to diaminophenothiazinium derivatives of formula (I); in which R, R, R, R, R, Rand Xare as defined in Claim, and also the methods for labelling oligonucleotides using such a derivative, labeling substrates and the oligonucleotides which can be obtained by means of such methods or from such labelling substrates.

Diaminophenothiazinium derivatives for labelling biomolecules, method and substrate for labelling oligonucleotides, and oligonucleotides obtained

The invention concerns a material composed of a porous support on which functionalized nanoparticles are grafted by covalent bonding, characterized in that at least part of the nanoparticles grafted by covalent bonding is housed inside surface pores of the support, and in that the support is silica-based and is in the form of porous particles of heterogeneous shape and size, the size of the particles being larger than 1 &#x3bc;m and preferably within the range of 5 to 200 &#x3bc;m. The invention further concerns a growth method for oligonucleotides or peptides characterized in that growth is performed on a material formed of a porous support on which functionalized nanoparticles are grafted by covalent bonding, characterized in that at least a part of the nanoparticles grafted by covalent bonding is housed inside surface pores of the support.

Material for supported synthesis and method for growing oligonucleotides or peptides

The invention relates to an electropolymerizable monomer, intended to be polymerized in aqueous solution, comprising a single electropolymerizable unit and an electron-donating group, characterized in that it also comprises at least one arm ionizable in aqueous solution. The invention also relates to the polymerization process, to the electroactive probe thus obtained and to the method for the detection of a target ligand in a biological sample.

Electropolymerisable monomers that are soluble in aqueous solution and electroactive probes that can be obtained with such monomers

The invention relates to novel pyrrole derivatives of the formula (I) which make it possible to immobilize and address oligonucleotides by electropolymerization. Said invention also relates to thus produced electroactive polymers and to methods for using them for detecting, identifying and dosing analytes in a sample. (I) wherein Ris one type of oligonucleotide, Y is S or O, X is a spacer arm selected from &#x2014;(CH)&#x2014;O&#x2014;, &#x2014;(CH)&#x3c;SB&#x3e;P&#x3c;/SB&#x3e;O&#x2014;[(CH)&#x2014;O]&#x2014;, &#x2014;(CH2); &#x2014;CO&#x2014;NR'&#x2014;(CH)&#x2014;O&#x2014;, &#x2014;CH&#x2014;NCH&#x2014;(CH)&#x2014;O&#x2014;, &#x2014;CH)CO&#x2014;NR&#x2032;&#x2014;[CH)&#x2014;O]&#x2014;, &#x2014;(CH)NCH[(CH)&#x2014;O]&#x3c;SB&#x3e;S&#x3c;/SB&#x3e;&#x2014;, R&#x2032; is H or CH, n is an integer number ranging from 1 to 5, p is an integer number ranging from 1 to 2, q is an integer number ranging from 1 to 4, r is an integer number ranging from 1 to 3, r&#x2032; is an integer number ranging from 1 to 3, s is an integer number ranging from 1 to 3, n, p, q, r, r&#x2032; and s are identical or different, a pyrrole cycle is substituted in a position 2, 3, 4 or 5.

Pyrroles substituted by oligonucleotides

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Chaponnay, France

Carole Chaix