E.i. Dupont De Nemours and Company

Alliance for Sustainable Energy, LLC

E.i. Du Pont De Nemours and Company Alliance for Sustainable Energy LLC

National Renewable Energy Laboratory

Carol M McCutchen

Paul Veikko Viitanen

Min Zhang

Yat-Chen Chou

Perry G Caimi

Vasantha Nagarajan

Michael G Bramucci

Luan Tao

Mary Ann Franden

Xu Li

Stuart M Thomas

Yuying Zhang

Laura McCole

Mark Emptage

Strains ofwere engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of theglyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Zymomonas with improved xylose utilization

Identified are mutants of the promoter of theglyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in, and other related bacteria.

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Production of ethanol using a strain of xylose-utilizingwith a genetic modification of the glucose-fructose oxidoreductase gene was found to be improved due to greatly reduced production of xylitol, a detrimental by-product of xylose metabolism synthesized during fermentation.

Ethanol production using xylitol synthesis mutant of xylose-utilizing zymomonas

A strain of xylose-utilizingwas engineered with a genetic modification to the glucose-fructose oxidoreductase gene resulting in reduced expression of GFOR enzyme activity. The engineered strain exhibits reduced production of xylitol, a detrimental by-product of xylose metabolism. It also consumes more xylose and produces more ethanol during mixed sugar fermentation under process-relevant conditions.

Xylitol synthesis mutant of xylose-utilizing zymomonas for ethanol production

This invention relates to a biocatalytic process to produce terephthalic acid and isophthalic acid from p-xylene and m-xylene, respectively. Terephthalic acid has been prepared by oxidizing p-xylene with bacteria belonging to the genus Burkholderia. Conversion of p-xylene into terephthalic acid is accomplished by a single bacterial strain that produces all of the requisite enzymes. In addition, this invention relates to the preparation of isophthalic acid from a mixture of m- and p-xylene.

Terephthalic acid producing proteobacteria

Microbial production of terephthalic acid and isophthalic acid

Growing community of inventors

Wilmington, DE, United States of America

Carol M McCutchen