UniversitÉ Libre De Bruxelles

La Region Wallonne

Laregion Wallone

Carol Deby

Alex Bollen

Joel Pincemail

Human myeloperoxidase produced by culturing prokaryotic or eukaryotic cells transformed by a vector for the expression of human myeloperoxidase in said cells.

Human myeloperoxidase and its therapeutic application

A method of preparing biologically active human myeloperoxidase including the steps of preparing a vector for the expression of human myeloperoxidase. The vector includes plasmid pNIV2703. The plasmid includes a HindIII-SnaBI, HindIII-EcoRV or HindIII-HpaI cassette, the cassette including a complete DNA sequence for hMPO as shown in FIG. 1. CHO cells are transformed with the vector. The cells are cultured. The biologically active human myeloperoxidase is recovered.

A substantially pure recombinant human myeloperoxidase heme-containing precursor, comprising a glycoprotein of 84 KD with the amino acid sequence coded for by the nucleotide sequence from 145 to 2235 corresponding to codons 49 to 745 in phase after the first methionine codon in FIG. 1 produced by culturing prokaryotic or eukaryotic cells transformed by a vector for the expression of human myeloperoxidase precursor in said cells.

Growing community of inventors

Liege, Belgium

Carol Deby