Novartis Vaccines & Diagnostics, Gmbh

Chiron Corporation

Carlos George-Nascimento

David Y Chien

Angelica Medina-Selby

Doris Guenzi Coit

Laura Tandeske

Philip J Barr

Dino Dina

Paul A Luciw

Phillip M Arcangel

Martha Truett

Deborah Lynn Parkes

Kathelyn S Steimer

Ray Sanchez Pescador

Rob Hallewell

Sansan Lin

Lin Sansan

Modified HCV multiple epitope fusion antigens (MEFAs) are described. The proteins include modified sequences such that proteolytic cleavage of the MEFAs by HCV NS3 protease is inhibited. HCV immunoassays including the modified MEFAs are also described.

HCV multiple epitope fusion antigens with modified proteolytic cleavage sites and uses thereof

Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.

Purified pol DNA, a recombinant DNA construct for expression of HIV pol polypeptide sequences and a cell containing such construct

Method for expressing HIV polypeptides

Recombinant DNA construct for HIV envelope polypeptides and polypeptides produced thereby

Human immunodeficiency virus (HIV) nucleotide sequences

The invention relates to a method of detecting HCV infection in a biological sample, the method comprising providing an immunoassay solid support, comprising an HCV anti-core antibody, an antigen comprising an HCV NS3/4epitope, and an HCV multiple epitope fusion antigen, that can detect both HCV antigens and antibodies present in a sample. The invention also includes polynucleotides encoding multiple epitope fusion antigens for use in the assay, recombinant vectors and host cells comprising such polynucleotides, and methods of producing the multiple epitope fusion antigens.

Polynucleotides encoding a multiple epitope fusion antigen for use in an HCV antigen/antibody combination assay

HCV immunoassays comprising an NS3/4a conformational epitope and a multiple epitope fusion antigen are provided, as well as immunoassay solid supports for use with the immunoassays.

Immunoassays for anti-HCV antibodies

Multiple fusion antigens for use in immunoassays for anti-HCV antibodies

An HCV core antigen and NS3/4a antibody combination assay that can detect both HCV antigens and antibodies present in a sample using a single solid matrix, is provided, as well as immunoassay solid supports for use in the assay.

HCV antigen/antibody combination assay

HIV immunoassays using gag polypeptides

Growing community of inventors

Walnut Creek, CA, United States of America

Carlos George-Nascimento