Dsm IP Assets B.v.

Dsm Sinochem Pharmaceuticals Netherlands B.v.

Carlos Enrique Lenhardt

Harold Monro Moody

Dennis Heemskerk

Theodorus Johannes Godfried Maria Van Dooren

Anja Gerarda Margaretha Hogenboom

Van Theodorus Johannes Godfried Maria Dooren

The present invention describes a process for preparing cephradine, said process comprising reacting 7-aminodesacetoxy cephalosporanic acid (7-ADCA) with D-dihydrophenylglycine in activated form (DHa) in the presence of an enzyme in a reaction mixture to form cephradine, resulting in a conversion of 7-ADCA into cephradine of at least 70%, wherein the concentration D-dihydrophenylglycine (DH) in the reaction mixture is below 2 wt.%, wherein the conversion of 7-ADCA into cephradine & equals; (n/n)*100%, wherein n=quantity of cephradine formed (in mole); and n=total quantity of 7-ADCA added to reaction mixture (in mole). The invention also describes a process for the preparation of cephradine hydrate characterised in that the process comprises: &#x2014;reacting 7-amino acid desacetoxy cephalosporanic acid (7-ADCA) with DHa in the presence of an enzyme in a reaction mixture to form cephradine; &#x2014;preparing an aqueous solution comprising at least part of the cephradine; and crystallising the cephradine from said aqueous solution. The invention further describes cephradine hydrate obtainable by a process according to invention. The invention also describes cephradine hydrate with an absorbance at 450 nm of below 0.050.

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Alella Barcelona, Spain

Carlos Enrique Lenhardt