Erasmus University Medical Center Rotterdam

Universidad De Salamanca

University of Salamanca

Carlos Eduardo Pedreira

José Alberto Orfao De Matos Correia E Vale

Jacobus Johannes Maria Van Dongen

Vincent Henricus Johannes Van Der Velden

Paulo Jorge Monteiro Da Silva Lucio

Sebastian Böttcher

Ester Mejstríková

Julia Maria Almeida Parra

Ludovic Bernard Simon Lhermitte

Vahid Asnafi

Tomasz Szczepanski

Juan Alejandro Flores-Montero

Andrew Craig Rawstron

Ruth Mary De Tute

Marta Martín Ayuso

Elaine Sobral Da Costa

Juan Alejandro Flores Montero

Ludovic Bernard Lhermitte

Marta Marin Ayuso

Jacobus Johannes Van Dongen

Vincent Henricus Van Der Velden

Marta Marín Ayuso

Alberto Orfao De Matos Correia E Valle

Sebastian Bottcher

Sebastian Boettcher

Jose Alberto Orfao De Matos Correia E Vale

The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least there identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

Methods, reagents and kits for flow cytometric immunophenotyping

The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

A method for detecting aberrant phenotypes expressed by neoplastic cells includes the steps of: 1) staining one or more normal/reactive samples and one neoplastic sample with multiple combinations of monoclonal antibodies, 2) measuring fluorescence emissions associated to the stained cells, 3) storing two independent list mode data files of information on light scatter and fluorescence characteristics of each cell, 4) creating new data files by mixing list mode data from the data file containing information the neoplastic sample into the data file containing information on the normal samples, 5) defining corresponding to normal cells and areas corresponding to empty spaces in normal/reactive samples that may be occupied by tumor cells in neoplastic samples, 6) identifying events corresponding to neoplastic cells and events corresponding to normal cells coexisting in a multidimensional space, and 7) establishing the most relevant phenotypic aberrations displayed by the neoplastic cells as compared to their normal counterpart.

Multidimensional detection of aberrant phenotypes in neoplastic cells to be used to monitor minimal disease levels using flow cytometry measurements

A method that generates new flow cytometry data files with a potentially infinite number of dimensions is described. First two or more separate flow cytometry data files containing information about events measured in different aliquots from the same sample are fused. Then, the magnitude of the values and a corresponding uncertainty measure of the differing variables between one or more of the original data files which have been fused are estimated, for each individual event contained in the fused data file, for which that variable was not directly measured in the flow cytometer. The new data file containing both data actually measured in the flow cytometer for each event of the individual original data files fused and the estimated data for those parameters not measured in a group of events from one or more of the original data files are reconstructed multidimensionally.

Method for generating new flow cytometry data files containing an infinite number of dimensions based on data estimation

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Rio de Janeiro, Brazil

Carlos Eduardo Pedreira