Columbia University

Amersham Biosciences Ab

Carl Yamashiro

Robert Elghanian

Frederic Zenhausern

Kenneth Luehrsen

Vineet Gupta

Abhijit Mazumder

Ralf Lenigk

Sally A Amundson

Chang-Gong Liu

Michael L Bittner

Ramesh Ramakrishnan

Arkadiy Silbergleyt

Todd Tuggle

Yoganand Balagurunathan

Mark Richards

Ekaterina Pestova

David R Fermin

David G Wang

Handy Yowanto

Zhijie John Gu

Krishnamurthy Jayaraman

Buena Chui

Christine Orozco

Gretchen Kiser

Changming Li

Exposure to ionizing radiation can produce a well-defined dose dependent signature in terms of changes in gene expression. In approaches and devices described herein, such a signature can be used to generate and use a self-contained radiation biodosimeter device, based on, for example, a blood finger stick. Various aspects of the invention are directed to biodosimetry with a fully integrated biochip using gene expression signatures.

Systems and methods for biodosimetry with biochip using gene expression signatures

This invention relates to methods and compositions for determining single nucleotide polymorphisms (SNPs) in P450 genes. In preferred embodiments, self extension of interrogation probes is prevented by using novel non self-extension probes and/or methods, thereby improving the specificity and efficiency of P450 SNP detection in target samples with minimal false positive results. The invention thus describes a variety of methods to decrease self-extension of interrogation probes. In addition, this invention provides a unique collection of P450 SNP probes on one assay, primer sequences for specific amplification of each of the seven P450 genes and amplicon control probes to evaluate whether the intended p450 gene targets were amplified successfully. The invention also describes a variety of array platforms for performing the assays of the invention; for example: CodeLink&#x2122;, eSensor&#x2122;, multiplex arrays with cartridges etc., all described herein.

P450 single nucleotide polymorphism biochip analysis

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Chandler, AZ, United States of America

Carl Yamashiro