Pioneer Hi-bred International, Inc.

Monsanto Technology LLC

Pioneer Hi-bred Int'l Inc.

Pioneer Hi-bred, Inc.

Carl W Garnaat

Marc C Albertsen

Bradley Allen Roth

Catherine D Brooke

Paul C C Feng

Gary A Huffman

Rebecca A Kelly

Jintai Huang

Youlin Qi

Timothy W Fox

Martin A Stoecker

Oscar Heredia

Agustin E Fonseca

Terry EuClaire Meyer

Timmy L Kendall

Paul C Feng

Keith S Lowe

Wesley B Bruce

Matthias Liebergesell

Steven W Ritchie

Jerome P Ranch

Terry Euclaire Meyer

Paul CC Feng

The invention provides transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products derived from event MON 87427. The invention also provides nucleotides specific for transgenic maize event MON 87427 and plants, plant cells, seeds, plant parts, and commodity products comprising nucleotides specific for transgenic maize event MON 87427. The invention also provides methods related to transgenic maize event MON 87427 and to the Roundup&#xae; Hybridization System (RHS). The invention also provides a Relative Development Scale useful for monitoring and determining reproductive development in maize that reconciles developmental differences across various maize varieties. This is useful for determining the optimal timing of a treatment regimen in which tassel development stage is an important factor, including various methods in making hybrid seed.

Transgenic maize event MON 87427 and the relative development scale

Auxotrophicand methods employing auxotrophicare provided. Auxotrophicmay be used in a variety of methods including biologically containingcomprising a transgene and transforming a plant cell without using ancounter-selective agent. Transforming maize immature embryos using anauxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic. Methods for producing anthymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.

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The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone or ultraspiracle receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor and a novel ultraspiracle receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.

Ecdysone receptors and methods for their use

The present invention relates to isolated male tissue-preferred regulatory sequences from Ms45 gene. The invention also relates to the use of Ms45 gene male tissue-preferred regulatory sequences in mediating male fertility in a plant, and in producing hybrid seed. The Ms45 male tissue-preferred regulatory region can be operably linked to exogenous genes encoding cytotoxins or inhibitory molecules. The invention also relates to plant cells, plant tissue and plants that contain the MS45 gene regulatory sequences.

Male tissue-preferred regulatory sequences of Ms45 gene and method of using same

The invention provides isolated auxin-responsive transcriptional regulatory elements and polynucleotides associated therewith. The present invention provides methods and compositions related to altering gene expression in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

Austin responsive promoter sequences and methods of using the same

The present invention provides compositions and methods for regulating the expression of nucleotide sequence of interest in a plant. Compositions include a novel nucleotide sequence comprising an inducible promoter for the 5C9 gene, which encodes a maize patatin-like polypeptide. A method for expressing a nucleotide sequence of interest in a plant using the promoter sequences disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the inducible promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence in an inducible manner.

Inducible promoters

The field of the invention is inducible gene expression systems wherein expression is controlled by a novel ecdysone receptor or its derivatives. The field particularly relates to the isolation and characterization of nucleic acid and polypeptides for a novel ecdysone receptor. The nucleic acid and polypeptides are useful in novel gene expression systems inducible with ecdysone or derivative receptor agonists.

The present invention relates to an isolated nucleic acid sequence encoding the Ms45 male tissue-preferred regulatory region. In one aspect this invention relates the use of this male tissue-preferred regulatory region in mediating fertility. An example of such use is the production of hybrid seed such as in a male sterility system. The Ms45 male tissue-preferred regulatory region can be operably linked with exogenous genes, such as those encoding cytotoxins, complementary nucleotidic units and inhibitory molecules. This invention also relates to plant cells, plant tissue and differentiated plants which contain the regulatory region in this invention.

Male tissue-preferred regulatory region and method of using same

Nucleotide sequences encoding a maize 5C9 polypeptide, the 5C9 polypeptide and structural and functional equivalents, and methods for controlling insect infestation in plants are provided.

Maize protein for insect control

Maize gene and protein for insect control

Growing community of inventors

Ankeny, IA, United States of America

Carl W Garnaat