University of Utah Research Foundation

Idaho Technology, Inc.

Biofire Defense, LLC

Biofire Diagnostics, LLC

Other

Canon U.s.a., Inc.

Utah Valley University

Roche Diagnostics Gmbh

Canon U.S. Life Sciences, Inc.

Kirk M. Ririe

Carl T. Wittwer

Randy P. Rasmussen

Carl Thomas Wittwer

Luming Zhou

Virginie E Dujols

Kirk M Ririe

Randy P Rasmussen

Philip Seth Bernard

David R Hillyard

Robert Andrew Palais

Gudrun Reed

Jared Steven Farrar

Bruce Kent Gale

Mark Aaron Poritz

Gregory Pritham

Martin Gutekunst

Sabine Lohmann

Niel Davenport Crews

Lindsay N Sanford

C Wade Dummer

Joseph A Holden

Carlynn Willmore-Payne

Jian Feng Chen

David E Jones

Jian Chen

Ivor T Knight

Brian E Caplin

Richard David Abbott

Thomas Charles Robbins

Scott O Sundberg

Jeanette Yang Paek

David J Eyre

Noriko Kusukawa

Cameron N Gundry

Wade R Stevenson

John Francis Quackenbush

Jessica Anne Houskeeper

Marla Lay

Andrew O Crockett

Steven Jared Farrar

Mark G Herrmann

Harold P Swerdlow

Mark G Herrman

Joseph T Myrick

Derek Allen David

Robert J Pryor

Charles W Dummer

Robert Palais

Lori A Wagner

Wade Stevenson

Robert A Palais

David Jones

Jospeh Holden

Virginie Dujols

Cameron Gundry

Methods, devices, and kits are provided for performing PCR in &#x3c;20 seconds per cycle, with improved efficiency and yield.

Methods for fast nucleic acid amplification

A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50&#xb0; C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.

High speed nucleic acid melting analysis

Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Nucleic acid melting analysis with saturation dyes

A method of background removal from melting curves generated using a fluorescent dye is provided for analyzing a melting profile of a nucleic acid sample. The method comprises measuring the fluorescence of the nucleic acid sample as a function of temperature to produce a raw melting curve having a melting transition, the nucleic acid sample comprising a nucleic acid and a molecule that binds the nucleic acid to form a fluorescently detectable complex, the raw melting curve comprising a background fluorescence signal and a nucleic acid sample signal; and separating the background signal from the nucleic acid sample signal by use of a quantum algorithm to generate a corrected melting curve, the corrected melting curve comprising the nucleic acid sample signal.

Quantum method for fluorescence background removal in DNA melting analysis

Methods, devices, and kits are provided for performing PCR and other thermal cycling reactions in &#x3c;20 seconds per cycle, using induction heating.

Induction PCR

Methods, kits and mixtures are provided for performing RT-PCR with an RT incubation of no more than one minute and PCR cycles in &#x3c;20 seconds per cycle.

Extreme reverse transcription PCR

Methods and kits for performing polymerase chain reaction (PCR) and melting analyses to determine copy number variation and/or gene expression are disclosed herein. Related uses of such methods and analyses are also disclosed herein.

Methods of detecting copy number variation

Devices are provided for performing polymerase chain reaction (PCR).

Methods are provided for fast nucleic acid amplification in which concentrations of primers and polymerase are increased.

Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Amplicon melting analysis with saturation dyes

Systems, methods, and kits are provided wherein a temperature-sensitive reagent that emits a luminescent signal is used to adjust the identification of the temperature of a sample or to control thermocycling. In various illustrative embodiments, the sample is a PCR mixture.

Monitoring temperature with fluorescence

Methods and kits are provided for nucleic acid analysis. In an illustrative method a target nucleic acid is amplified using a first primer and a second primer, wherein the first primer comprises a probe element specific for a locus of the target nucleic acid and a template-specific primer region, and the probe element is 5' of the template-specific primer region, subsequently allowing the probe element to hybridize to the locus to form a hairpin, generating a melting curve for the probe element by measuring fluorescence from a dsDNA binding dye as the mixture is heated, wherein the dye is not covalently bound to the first primer, and analyzing the shape of the melting curve. Kits may include one or more of the first and second primers, the dsDNA binding dye, a polymerase, and dNTPs.

Primers for melting analysis

Growing community of inventors

Salt Lake City, UT, United States of America

Carl Thomas Wittwer