Sienna Biotech, Inc.

Becton Dickinson and Company Limited

Qiagen Gaithersburg Inc.

Carl Theodore Edens

W Peter Hansen

Joseph L Katz

Jonathan Matthew Miller

Bert Jungheim

Andrew Leonard

Jiulin Xia

Bowen Cui

Nadia P Allen

Michael Cennerazzo

Manish S Kochar

Richard L Mantefuel

Peter Haviernik

Brian Austin Self

Disclosed are an optical flow particle apparatus and method for conducting a particle light scatter-based immunoassay for simultaneously measuring the presence or amount of one or more analytes in a fluid sample which involves the use of a reagent set for each analyte including first binding molecule-coated monodisperse microspheres and second binding molecule-coated colloidal particles in which at least one of the first or second binding molecules specifically binds a respective one of the analytes. In the case where more than one analytes are detected, each monodisperse microperse microsphere of a particular reagent set has a light scatter signal resolvable from that of microspheres of any other reagent set. Changes determined in the distributions of the measured light scatter signals for individual microspheres of each of the particular reagent sets are indicative of the presence or amount of the respective analyte(s) in the sample.

Light scatter-based immunoassay

A pre-analysis chamber having reduced turbulence is provided for a flow particle analyzer. The chamber has a conical diverging section for decelerating sheath fluid flow to form a laminar profile, followed by a conical converging section for accelerating flow. A sample inlet tube is supported in the chamber near the interface between the diverging and converging sections, and terminates in the laminar, accelerated sheath flow. The sample enters the laminar sheath flow to form a discrete sample stream.

Pre-analysis chamber for a flow particle analyzer

Growing community of inventors

Columbia, MD, United States of America

Carl Theodore Edens