E.i. Dupont De Nemours and Company

Carl A Maxwell

Stephen M Allen

Saverio Carl Falco

Brian McGonigle

William Dean Hitz

Omolayo O Famodu

Karlene H Butler

Yong Tao

Gregory T Bryan

Aideen Oonagh Hession

This invention is in the field of plant molecular biology. More specifically, this invention pertains to compositions having increased levels of at least one phytosterol, said compositions being obtained from plants or plant parts, and methods thereof. The plants may have decreased levels of triterpene saponins. The plants or plant parts may comprise at least one recombinant DNA molecule comprising a promoter operably linked to at least a portion of at least one polynucleotide from at least one oxidosqualene cyclase gene, said recombinant DNA molecule sufficient to increase the production of at least one phytosterol; or any progeny of said plant, wherein said progeny comprise said recombinant DNA molecule. Compositions, oils, as well as food and feed products obtained from or prepared with said compositions are also part of the invention.

Compositions with increased phytosterol levels obtained from plants with decreased triterpene saponin levels

This invention relates to an isolated nucleic acid fragment encoding an oxidosqualene cyclase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the isolated polynucleotides of the invention, in sense or antisense orientation, operably linked to a suitable regulatory sequence.

Enzymes involved in triterpene synthesis

This invention relates to an isolated nucleic acid fragment encoding a sulfate assimilation protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sulfate assimilation protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the sulfate assimilation protein in a transformed host cell.

Genes encoding sulfate assimilation proteins

This invention relates to an isolated nucleic acid fragment encoding a magnesium chelatase subunit. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the magnesium chelatase subunit, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the magnesium chelatase subunit in a transformed host cell.

Magnesium chelatase

This invention related to an isolated nucleic acid fragment encoding a tryptophan biosynthetic enzyme. This invention also related to the construction of a Chimeric gene encoding all or a portion of the tryptophan biosynthetic enzyme, in sense or antisense orientation, wherein expression of the Chimeric gene results in the production of altered levels of the tryptophan biosynthetic enzyme in a transformed host cell. The tryptophan biosynthetic enzymes include anthranilate phosphoribosyltransferase (E.C. 2.4.2.18) from corn ( ), soybean ( ) and wheat ( ); indole-3-glycerol phosphate synthase (E.C. 4.1.1.48) from corn ( ), rice ( ), soybean ( ), and wheat ( ); and phosphoribosylanthranilate isomerase (E.C. 5.3.1.24) from corn ( ), rice ( ), (and wheat ( ).

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Elkton, MD, United States of America

Carl A Maxwell