Cornell Research Foundation Inc.

Cornell University

Carl A Batt

David Erickson

Adam Joseph Lowe

Yun Suk Huh

Richard N Brandon

Martin Wiedmann

A method for detecting target nucleic acids such as SNPs is provided. The method comprises performing a ligase detection reaction (LDR), performing surface enhanced Raman scattering (SERS) on the LDR, and analyzing the outcome of the LDR using analysis and/or quantification of the SERS by detecting an emitted Raman signature. The LDR-SERS method can be used for sensitive and specific detection of any nucleic acid sequence of interest. A microfluidic SERS detection device is also provided. The device comprises electrokinetically active microwells for mixing and concentrating analytes and in which analytes can be quantified. The device can be used for performing the LDR-SERS method in optofluidic chip format.

Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells

The present invention relates to novel compositions comprising Bovine Herpesvirus-1 (BHV-1) specific oligonucleotides which are useful as nested primers to amplify sequences of the BHV-1 gIV gene during enzymatic nucleic acid amplification. The invention also provides a method for the detection of BHV-1 which may be present in a clinical specimen, particularly bovine semen, using the BHV-1 specific nested primers and enzymatic nucleic acid amplification. The present invention also relates to a BHV-1 specific oligonucleotide which can be used as a probe to facilitate detection of amplified products derived from BHV-1 gIV gene sequences.

Methods of detecting bovine herpesvirus 1 (BHV-1) in semen by nucleic

Mouse monoclonal antibodies which will specifically recognize the pathogen Listeria monocytogenes were produced by fusion of spleen cells from an animal immunized with live L. monocytogenes to an NS-1 myeloma partner, and three hybridomas were identified upon subsequent subcloning, Mab 20-10-2, Mab 36-6-12 and Mab 56-9-16 which were preferentially reactive with L. monocytogenes in a direct binding ELISA assay. An indirect 'sandwich' assay was developed and used to further confirm the reactivity of these hybridomas using four serotypes of L. monocytogenes and other common cross reacting bacteria.

Growing community of inventors

Groton, NY, United States of America

Carl A Batt