The United States of America, As Represented by the Secretary, Department of Health and Human Services

2020 Genesystems Inc.

Capre Denise Mitchell

Michael R Emmert-Buck

Stephen M Hewitt

Dan-Paul Hartmann

Vladimir Knezevic

Galina Baibakova

Kevin Gardner

The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section (such as a fresh or archival sample, tissue microarray, or cells harvested by an LCM procedure) or other substantially two-dimensional sample (such as an electrophoretic gel or cDNA microarray) by creating 'carbon copies' of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules (e.g., proteins and nucleic acids) in the samples. Other specific methods are provided for the identification and analysis of proteins and other biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes that can be used in described methods, and which permit the identification and analysis of biomolecules.

Methods, devices, arrays and kits for detecting and analyzing biomolecules

Growing community of inventors

Winston Salem, NC, United States of America

Capre Denise Mitchell