Nanjing Forestry University

Caoxing Huang

Qiang Yong

Chenhuan Lai

Ke Wang

Yong Xu

Yuheng Tao

Xiaoxue Zhao

Yongcan Jin

A method for characterizing the interaction force between lignin and cellulase is provided. The method includes the following steps: (1) preparing a lignin solution, then dropwise adding the solution onto a silicon wafer, repeatedly spin-coating with a spin coater, and vacuum-drying the prepared lignin film; (2) modifying an AFM probe coated with a gold film on the surface with HS-PEG-COOH and then activating the carboxyl group, immersing the AFM probe after the carboxyl group activation in a PBS solution of cellulase for incubation, and after the modification is completed, placing the washed AFM probe in a PBS buffer solution for storage for later use; (3) testing the lignin-cellulase interaction force by using an AFM instrument in a liquid phase environment.

Method for characterizing interaction force between lignin and cellulase

A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and() as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of β-mannanase and α-galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.

Method and special complex enzyme for hydrolyzing galactomannan (GM) to prepare small-molecule GM and galactomannan oligosaccharide (GMOS)

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Nanjing, China

Caoxing Huang