Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Cao Guo Yu

Andre R Abad

Billy Fred McCutchen

Ronald D Flannagan

James F Wong

Hua Dong

Xiaomei Shi

Sue B Lo

Bin Hu

Xiaofeng Sean Yang

Compositions and methods for protecting a plant from an insect pest are provided. In particular, novel polynucleotides and the pesticidal polypeptides they encode are provided. Methods of using the novel polynucleotides and pesticidal polypeptides of the invention to protect a plant from an insect pest are further provided. Particular embodiments of the invention provide pesticidal compositions and formulations, DNA constructs, and transformed plants, plant cells, and seeds.

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The invention provides nucleic acids, and variants and fragments thereof, obtained from strains ofencoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal lipase gene products that are useful in the practice of this invention are also provided. The compositions and methods of the invention may be used in a variety of agricultural systems for controlling pests, including propagating lineages of insect-resistant crops and targeting expression of these insecticidal lipases to plant organs that are particularly susceptible to infestation, such as roots and leaves.

Methods of using non-plant insecticidal lipase encoding nucleic acids in transgenic plants

Methods and compositions for identifying novel pesticidal gene homologues are provided. Specifically, the methods of the invention comprise systematically designing oligonucleotide primers that are specific for a pesticidal gene of interest and performing successive rounds of PCR amplification of nucleic acid material from a microorganism, particularly astrain, to identify novel homologues of known pesticidal genes. Oligonucleotide primers that can be used to practice the present methods are further disclosed.

Methods for identifying novel pesticidal gene homologues

Methods of using non-plant lipase encoding nucleic acids

Methods of using non-plant encoding nucleic acids

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Irvine, CA, United States of America

Cao Guo Yu