Lonza Walkersville, Inc.

Other

Candice Stumbaugh

Kenneth E Nichols, Jr

Karen Lin

Leben Tadesse

David S Herbst

Kenneth E Nichols

The present invention is related to compositions comprising clarified limulus amebocyte lysate (LAL), wherein the LAL is substantially free of coagulogen and methods of making such compositions. The invention further relates to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising: (a) contacting the sample with a reagent comprising clarified LAL and a chromogenic substrate; and (b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample, wherein the LAL is substantially free of coagulogen. The invention also relates to kits comprising clarified LAL substantially free of coagulogen, and methods of making such.

Coagulogen-free clarified limulus amebocyte lysate

The present invention is related to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising: (a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate; and (b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample; wherein the LAL is substantially free of coagulogen. The method also relates to compositions and kits comprising LAL substantially free of coagulogen, and methods of making such.

Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen

Coagulogen-free clarified limulus amebocyte lysate and chromogenic assay of endotoxin

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Chambersburg, PA, United States of America

Candice Stumbaugh