Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Camille Deforceville

Thomas Bordy

Olivier Cioni

Ondrej Mandula

A method for analyzing microorganisms arranged in a sample is provided, the sample including a viability marker to modify an optical property of the microorganisms in different ways depending on whether they are dead or alive, the method including illumination of the sample and acquisition of an image of the latter by an image sensor, the image sensor then being exposed to an exposure light wave; determining positions of different microorganisms from the acquired image; applying a propagation operator to calculate at least one characteristic value of the exposure light wave at each radial position and at a plurality of distances from the detection plane representing a change in the characteristic value between the image sensor and the sample; and identifying living microorganisms according to each profile.

Method for analysing microorganisms

Method for observing a sample comprising the steps of (a) illuminating the sample using a light source, the light source emitting an incident light wave that propagates toward the sample along a propagation axis (Z); (b) acquiring, using an image sensor, an image of the sample, which image is formed in a detection plane; (c) forming a stack of images, called reconstructed images, from the image acquired in step (b), each reconstructed image being obtained by applying, for one reconstruction distance, a numerical propagation operator; and (d) from each image of the stack of images, computing a clearness indicator for various radial positions, each clearness indicator being associated with one radial position and with one reconstruction distance.

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Grenoble, France

Camille Deforceville