Ge Healthcare Bio-sciences Ab

Cytiva Sweden Ab

Camilla Estmer-Nilsson

Klaus Gebauer

Ralph Stankowski

Andreas Castan

Anders Wilen

Thomas Falkman

Michael Miller

Andreas Axen

John Clachan

Lena Maria Sandberg

Inger Andersson

Peder Bergvall

The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.

Bag assembly for cultivation of cells

The present invention relates to a method of preparing a separation matrix comprising at least one insoluble carrier to which sulphate ligands have been attached via extenders, which method comprises coupling, in a first step, of the sulphate ligands to the extenders and, in a subsequent step, attaching the extenders to an insoluble carrier. The invention also relates to a separation matrix comprised of at least one insoluble carrier to which sulphate ligands have been attached via extenders. Advantageously, no sulphate ligands are directly attached to the insoluble carrier. The extenders may be natural polymers, such as dextran, and the insoluble carrier may be made from natural polymers, such as agarose, or synthetic polymers. The invention also relates to a method of purifying virus, such as influenza virus, using a separation matrix according to the invention.

Separation matrix for viral purification

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Uppsala, Sweden

Camilla Estmer-Nilsson