Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Camilla Estmer Nilsson

Johan William Ohman

Martin Hall

James Van Alstine

Rolf Hjorth

Jamil Shanagar

Daniel Leif Ivansson

Therese Graner

Provided are methods for protein analysis in which proteins to be analyzed are displayed on a population of discrete and dispersible structures, such as beads or other particles, for subsequent affinity reactions and analysis/detection. The proteins to be analyzed may be provided as denatured protein samples in the presence of a denaturing agent.

Method for protein analysis

The present invention relates to a simple and efficient method to isolate and purify nucleic acids, preferably genomic DNA, from complex samples compared with available methods, by using a ligand which relies on hydrogen bonding to purify the nucleic acids. Preferably the ligand is bound to magnetic beads/particles. More closely the method comprises adding a sample comprising nucleic acid to a polymer having neutral charge; reversibly binding said nucleic acid to said polymer by hydrogen bonding under pH conditions &#x3c;5; washing said polymer; and eluting said nucleic acid from said polymer under conditions of pH &#x3e;5. The method is very suitable for sample preparation of nucleic acids, for example for PCR applications.

Purification of nucleic acid

The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase. With minor or no modification the target containing phase solution can be further processed via standard unit operations such as precipitation, chromatography, and filtration to further purify target and remove any residual polymer.

Separation method using single polymer phase systems

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Uppsala, Sweden

Camilla Estmer Nilsson