Harvard College

Massachusetts Institute of Technology

The Broad Institute, Inc.

The General Hospital Corporation

Cameron Myhrvold

Feng Zhang

Peng Yin

Omar O Abudayyeh

Jonathan S Gootenberg

James Joseph Collins

Paul Blainey

Deborah Hung

Pardis Sabeti

Catherine Amanda Freije

Eric S Lander

Ralf Jungmann

Mingjie Dai

Cheri Ackerman

Yonggang Ke

Omar Abudayyeh

Diming Wei

Dongran Han

Gowtham Thakku

The present invention offers a new approach for highly multiplexed, programmable antiviral therapies that directly target viral RNA, and can be flexibly adapted to target novel viruses or emerging outbreak pathogens. Class 2, type VI CRISPR system-based therapies can be used in combination with existing antiviral compounds for viruses where such compounds exist, either by increasing their efficacy or by preventing the evolution of specific drug resistance mutations. Perhaps most excitingly, if a virus evolves resistance to a specific guide RNA sequence, it is easy to switch to a different guide RNA sequence, or to design a new guide sequence to target the new mutation. Such approaches should prevent the widespread development of resistance to Class 2, type VI CRISPR system-based therapies.

CRISPR system based antiviral therapy

Provided herein is a nucleic acid detection system comprising: a CRISPR system comprising an effector protein and one or more guide RNAs designed to bind to corresponding target molecules; an RNA-based masking construct; and optionally, nucleic acid amplification reagents to amplify target RNA molecules in a sample. In another aspect, the embodiments provide a polypeptide detection system comprising: a CRISPR system comprising an effector protein and one or more guide RNAs designed to bind a trigger RNA, an RNA-based masking construct; and one or more detection aptamers comprising a masked RNA polymerase promoter binding site or a masked primer binding site. In some embodiments, the system may be used to detect viruses in samples.

CRISPR effector system based diagnostics for virus detection

The present disclosure relates to nanostructures assembled from nucleic acid consisting of a single strand of DNA rationally-designed to self-assemble into a hairpin loop, helical domains, and locking domains.

Single-stranded DNA nanostructures

The invention provides SST motifs of controlled size and shape, comprised of a plurality of oligonucleotides, and methods for their synthesis. The motifs are formed, at least in part, by the self-assembly of single stranded oligonucleotides into curved, corrugated or twisted structures. The location of each oligonucleotide in the resultant motif is known. Accordingly, the motifs may be modified with specificity.

Compositions and methods relating to complex nucleic acid nanostructures

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Cambridge, MA, United States of America

Cameron Myhrvold