Johnson & Johnson Clinical Diagnostics, Inc.

Eastman-kodak Company

Clinical Diagnostic Systems, Inc.

Calvin Roman Messing

Daniel S Daniel

David A Hilborn

Ignazio S Ponticello

Susan J Danielson

Wayne W Weber, Ii

Margaret Elizabeth Logan

Diaza-18-crown-6 compounds of the formula I: ##STR1## wherein X is a phenol, naphthol or quinolinol moiety selected from those of the formulae: ##STR2## R.sub.1, R.sub.2, R.sub.3, R.sub.4 and R.sub.5 are independently selected from hydrogen, halo, cyano, nitro, (C.sub.1 -C.sub.6) alkyl, (C.sub.2 -C.sub.8) acyl, acetamido, mercapto, alkyl or arylsulfonyl, trifluoromethyl, aryl, and substituted aryl wherein the aryl moiety is selected from phenyl and naphthyl and the aryl substituent is selected from halo, cyano, nitro, (C.sub.1 -C.sub.6) alkyl, (C.sub.2 -C.sub.8) acyl, phenyl, acetamido, mercapto, alkyl or arylsulfonyl and trifluoromethyl; provided that when R.sub.1, R.sub.2, R.sub.3 and R.sub.5 are hydrogen and X is phenol, R.sub.4 is not nitro; and provided that when X is phenol and R.sub.5 is hydrogen, R.sub.1 (or R.sub.2) and R.sub.4 are not both nitro. Compounds of the above formula are useful as chromoionophores for the detection of calcium ions in solution or dry assays, but are of particular advantage when used in dry, thin-film multilayer analytical elements.

Calcium specific diaza-18-crown-6-ether chromoionophores

A dry immunoassay analytical element, for assaying a ligand, comprising in the following order:

Immunoassay elements comprising polymers containing vandium IV (V+4) ions

A dry immunoassay analytical element, for assaying a ligand, comprising a support bearing:

Immunoassay analytical elements containing vanadium IV(V.sup.+4)ions

Photographic recording material is described which employs a combination of a mercury compound and a benzothiazolium compound to reduce chemical fog without adverse loss of photographic speed.

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Spencerport, NY, United States of America

Calvin Roman Messing