Juno Therapeutics Gmbh

Calvin Chan

Mark L Bonyhadi

Pascal Beauchesne

Chris Ramsborg

Brian Christin

Rachel K Yost

Ruth Berry

Janelle Stoops

Kien Khuu-Duong

Kien Khuu-duong

The present disclosure relates in some aspects to methods, cells, and compositions for preparing cells and compositions for genetic engineering and cell therapy. Provided in some embodiments are streamlined cell preparation methods, e.g., for isolation, processing, incubation, and genetic engineering of cells and populations of cells. Also provided are cells and compositions produced by the methods and methods of their use. The cells can include immune cells, such as T cells, and generally include a plurality of isolated T cell populations or types. In some aspects, the methods are capable of preparing of a plurality of different cell populations for adoptive therapy using fewer steps and/or resources and/or reduced handling compared with other methods.

Methods for isolating, culturing, and genetically engineering immune cell populations for adoptive therapy

Provided herein are methods of assessing or determining the presence or absence of particles, such as bead particles, present in a cell composition. Also provided are articles of manufacture and kits for use in the methods.

Methods of enumerating particles present in a cell composition

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Seattle, WA, United States of America

Calvin Chan