Cognis Corporation

Other

Cognis IP Management Gmbh

C Ron Wilson

David L Craft

L Dudley Eirich

Maria Tang

Krishna M Madduri

John C Loper

Yeyan Zhang

Mark Eshoo

Cathy A Cornett

Alfred A Brenner

Martin Anthony Gleeson

Dudley Eirich

Manfred Biermann

Jeffrey B Stavenhagen

Kevin W Anderson

Jeffrey A Gates

Gilbert H Vice

A bioprocess for producing carboxylic acids, alcohols and aldehydes is provided by culturingsp. in a fermentation medium containing various defined substrates.

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The present invention provides modified promoters fromCYP and POX4 genes. The modified promoters have various sequence motifs added, deleted, or altered in order to modulate expression of a coding sequence operably linked thereto. The sequence motifs comprise repressors of gene induction (URS sequences) and activators of gene induction (UAS sequences) as well as oleic acid response elements (ORE sequences). Yeast host cells comprising such modified promoters are also provided. Methods of altering expression of a protein of the beta or omega oxidation pathways using a subject modified promoter are also provided.

The present invention provides antigenic peptides useful for the production of antibodies which selectively bind to an enzyme involved in the &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids in yeast. Antibodies which specifically bind to an enzyme involved in the &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids in yeast are also provided. In addition, methods of producing the subject antibodies, a method of detecting the presence and amount of a specific enzyme involved in the &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids, and a method of monitoring the degree of enzyme induction and/or enzyme stability in a mixture during &#x3c9;-oxidation of fatty acids and alkanes to &#x3b1;,&#x3c9;-dicarboxylic acids in yeast, are also provided.

Antibodies for enzymes of the &#x3c9;-oxidation pathway and methods relating thereto

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &#x3c9;-hydroxylase complex of20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.

Method of increasing conversion of a fatty acid to its corresponding dicarboxylic acid

Use of CYP52A2A promoter to increase gene expression in yeast

Novel genes have been isolated which encode cytochrome P450 and NADPH reductase enzymes of the &ohgr;-hydroxylase complex of,20336. Vectors including these genes, transfected host cells and transformed host cells are provided. Methods of producing of cytochrome P450 and NADPH reductase enzymes are also provided which involve transforming a host cell with a gene encoding these enzymes and culturing the cells. Methods of increasing the production of a dicarboxylic acid and methods of increasing production of the aforementioned enzymes are also provided which involve increasing in the host cell the number of genes encoding these enzymes. A method for discriminating members of a gene family by quantifying the expression of genes is also provided.

Cytochrome P450 monooxygenase and NADPH cytochrome P450 oxidoreductase genes and proteins related to the omega hydroxylase complex of Candida tropicalis and methods relating thereto

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Loveland, OH, United States of America

C Ron Wilson