The Penn State Research Foundation

Intrexon Corporation

Interexon Corporation

C Peter Romaine

Jack Q Wilkinson

Xi Chen

Zhixin Shi

Donald S Walters

Alan Marlowe

Michael A Sulzinski

Carl David Schlagnhaufer

Gary W Moorman

Benjamin Michael Woolston

The present invention provides compositions and methods for regulating expression of nucleotide sequences in fungi. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from thelectin gene. The sequences drive expression preferentially to fruit body tissue. A method for expressing a nucleotide sequence in fungi using the regulatory sequences disclosed herein is provided. The method comprises transforming a fungal cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed fungus from the transformed cell.

Regulatory element for heterologous protein production in the fruiting body of filamentous fungi

Disclosed is a new technology that enables the transgenic modification of a mushroom-forming fungus to confer a transgenic genotype and/or phenotype. According to the invention herein, one can manipulate the fruiting body of a fungus conferring an altered phenotype, but a wild-type genotype. The fruiting body itself is devoid of any cognate transgene, and Applicants have discovered a new mechanism of protein synthesis and accumulation in fungi and a technique to independently control the genotype and phenotype of the fruiting body.

Strategies for the transgenic manipulation of filamentous fungi

Applicants have devised a highly effective, convenient, and expeditious genetic transformation system for filamentous fungi, such as. The preferred method uses an-mediated transformation protocol. The critical features of this protocol include co-cultivation of the bacterium with fruit body tissue instead of spores. In a preferred embodiment, even higher transformation efficiencies were observed with the use of a homologous promoter in the polynucleotide expression construct in order to drive gene expression.

Methods and compositions for highly efficient transformation of filamentous fungi

This invention provides methods and reagents for screening plants, particularly geraniums, for infections with bacterial pathogens. Specifically, the invention provides nucleic acid amplification and detection methods for specifically detecting a certain pathovar of a bacterial infectious agent in geraniums. The invention provides a nucleic acid hybridization probe that is specific for Xanthomonas campestris pathovar pelargonii, and paired sets of oligonucleotide primers for polymerase chain reaction-catalyzed amplification of DNA fragments comprising the nucleic acid sequences of the invention specific for Xanthomonas campestris pathovar pelargonii template DNA. The invention also provides PCR-based methods for detecting Xanthomonas campestris pathovar pelargonii DNA, and in particular for distinguishing pathovar DNA from the DNA of the host infected plant. Methods for producing detectably-labeled hybridization probes using PCR are also provided.

Polymerase chain reaction-based diagnostic test for the detection of

There is provided a method of cultivating mushrooms and mushroom spawn, wherein a delayed-release nutrient supplement is utilized. The method is an improvement over prior methods which utilize nutrient supplements in the growing of mushrooms and mushroom spawn. The improvement comprises using as a nutrient supplement an intact seed having its sprouting capability irreversibly inhibited or destroyed, or mixtures thereof, and having inherent delayed-release properties.

Intact seed-based delayed-released nutrient supplement for mushroom

Intact seed of rapeseed is added to compost at spawning to increase the yield of mushrooms similar to chemical-based delayed-release supplements. No chemicals, however, are needed in its preparation, only a treatment to destroy sprouting of the seed.

Growing community of inventors

State College, PA, United States of America

C Peter Romaine