Johns Hopkins University

The United States of America As Represented by the Department of Health

C Brent Bargeron

Harvey Wayne Ko

Micah A Carlson

Terry E Phillips

Charles W Anderson

Allan B Fraser

Richard C Benson

David R Kohler

John D Groopman

Paul T Strickland

Owen J Deters

A hand held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined. The rinsing and elution fluids, and/or the affinity column can be placed in a module for easy insertion and removal from the sensor when depleted or when the sensor is to be used against a different analyte.

Miniature immuno-optical rapid analyte sensor platform

A laser Doppler velocimeter which can measure fluid flow in three dimensions, employing a laser beam projected through a rotating diffraction grating and spaced lenses, with a beam splitter in the major optical path between two lenses ahead of the flow channel through which the fluid passes. Selected pairs of laser-derived beams are directed through the fluid and intersect at a predetermined point in the fluid. The movement of the particles through interference patterns formed by the intersecting laser-derived beams generates further-modulated scattered light, which is focused on a photomultiplier tube, from the output of which a fluid velocity directional component signal is derived for each pair. There are four laser-divided beams, and different pairs of these beams can be selected, to provide measurements from which the components of the fluid velocity along three mutually orthogonal directions can be derived.

Three dimensional laser Doppler velocimeter

Growing community of inventors

Columbia, MD, United States of America

C Brent Bargeron