Doosan Corporation

Sunkyong Industries Ltd.

Doosan Technical Center

Sunhill Glucose Co., Ltd.

Byung Cheol Seo

Key Hyup Kim

Jung Ryul Kim

Yang D Choi

Kwan H Park

Jae H Cha

In C Kim

So Y Jang

Jin Byung Park

Jeoung R Kim

The present invention relates to a fermentation process for preparing erythritol with high productivity using Trichosporonoides madida DS 911, more specifically, for preparing erythritol by optimizing the culture conditions such as pH, temperature, aeration rate and agitation speed, and by developing the process of fed-batch culture such as feeding strategy of substrate and composition of feeding substrate.

Fermentation process for preparing erythritol using Trichosporonoides

An Escherichia coli (ATCC 68319) containing a gene encoding a novel maltogenic amylase of Bacillus licheniformis (BLMA), wherein said gene has a length of about 3.5 kb; said gene expresses a gene product capable of hydrolyzing cyclodextrin, pullulan, as well as starch at an optimum temperature of about 50.degree. C. at a pH of about 7; said gene product has sugar transferase activity in the presence of glucose; and said Escherichia coli produces said gene product optimally when grown in Luria broth, for 24 hours, at 37.degree. C.

Escherichia coli containing a gene encoding a maltogenic amylase BLMA of

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Seoul, South Korea

Byung Cheol Seo