Cj Corporation

Cj Cheiljedang Corporation

Cheiljedang Corp.

Byoung Choon Lee

Young Hoon Park

Jin Ho Lee

Dae Cheol Kim

Jae Yong Cho

Yong Uk Shin

Jae Yong Park

Kwang Myung Cho

Young Hoon 111-102 Mujigae Daerim Apt Park

Jae Yong 505-802 Manhyun Maeul Hyundai Cho

Young Hoon 111-102 Mujigae Daelim Apt Park

Dae C 201-302 Hwanggol Maeul Shinmyung Apt Kim

The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.

[object Object]

A method for producing L-threonine using a microorganism is provided. In the method, the threonine dehydratase (tdc) gene existing in the genomic DNA of the microorganism is partially deactivated using a recombination technique. For a microorganism strain with enhanced activity of threonine operon-containing enzymes and the phosphoenolpyruvate carboxylase (ppc) gene, the tdc gene engaged in one of the four threonine metabolic pathways is specifically deactivated, thereby markedly increasing the yield of L-threonine.

Method for L-threonine production

Provided are a microorganism capable of producing L-threonine and having an inactivated tyrR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.

Microorganism producing L-threonine having inactivated tyrR gene, method of producing the same and method of producing L-threonine using the microorganism

Provided are a microorganism capable of producing L-threonine and having an inactivated galR gene, a method of producing the same and a method of producing L-threonine using the microorganism. The microorganism can be used to produce L-threonine in high yield.

Microorganism producing L-threonine having inactivated galR gene, method of producing the same and method of producing L-threonine using the microorganism

An L-threonine-producingstrain and a method for producing the same are provided. Thestrain contains chromosomal DNA with inactivated metJ gene. Therefore, expression repression of threonine biosynthesis genes by a metJ gene product is prevented, thereby producing a high concentration of threonine. Further, a high concentration of L-threonine can be produced in high yield using the method.

Method for producing L-threonine

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Seoul, South Korea

Byoung Choon Lee