Other

Buz Barstow

Michael Baym

Lev Shaket

Isao Anzai

Oluwakemi Adesina

Systems and methods for isolating DNA molecules that can include the steps of: providing a transposon mutant collection, the transposon mutant collection being stored in a plurality of wells; dispatching aliquots from each well in the transposon mutant collection to a set of pools in a combinatorial pooling array that uses pool address coordinates, the aliquots being dispatched to the set of pools based on a location of the aliquots within the transposon mutant collection; constructing an amplicon library, the amplicon library including a sequencing dataset; parsing the sequencing dataset to a set of putative transposon insertion locations and pool address coordinates; calculating a likelihood for each location and pool address coordinates within the set of putative transposon insertion locations and pool address coordinates using a Bayesian inference algorithm informed by internal self-consistency; and generating a progenitor collection catalog based on location and pool address coordinates verified in the calculating step.

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Princeton, NJ, United States of America

Buz Barstow