Purdue Research Foundation

Bumsoo Han

George Tsu-Chih Chiu

Kinam Park

Thomas Heinrich Siegmund

Yumeng Wu

Crystal Soo Jung Shin

Cih Cheng

Matthew Tyler Short

Yingnan Shen

Michael Linnes

Bongseop Kwak

Disclosed herein is a platform and method to quantify spatiotemporal tissue swelling during biologics injection, and to predict associated increase in the mechanical stress and interstitial fluid pressure (IFP) of tissues. Accurate measure and estimation of tissue swelling, thus, can be quantitative and predictive indicator of the IPD.

Characterization of injection-induced tissue swelling during subcutaneous injection of biologics

The present disclosure relates to a novel method for rapid preparation of three dimensional (3D) spheroids/organoids, and the 3D spheroids/organoids prepared by the novel method.

Methods and materials for rapid preparation of 3D spheroids/organoids

Multi-well collapsible basket arrays and methods for their use with high throughput culture and histology analysis of spheroids and organoids. Such a collapsible basket array includes a collapsible cellular array structure having multiple cells and connectors that interconnect adjacent pairs of the cells to cause the collapsible cellular array structure to collapse from an expanded configuration to a collapsed configuration in which the connectors are partially wrapped around perimeters or circumferences of the cells, whereby the collapsible cellular array structure is expandable to acquire an expanded configuration capable of individually aligning the cells thereof with wells of a well plate. The collapsible basket array further includes inserts individually mountable to the cells of the collapsible cellular array structure, with each insert including a permeable basket with pores sized to retain spheroids or organoids within the basket.

Collapsible basket arrays, collapsible cellular arrays therefor, and methods of use

A system is provided that simulates the in vivo micro-environment of three-dimensional cellular structures or bodies, such as tumors. The system simulates the pressure gradients and fluid flows of the vascular and lymphatic systems as well as the interstitial and capillary transport mechanisms between the 3D cellular structure and the vascular and lymphatic systems. The system can be used to introduce drugs or drug delivery carriers to a tumor, for example, to assess the uptake capability and effect on the tumor. The system maintains the viability of the tumor cells for a sufficiently long period of time to permit testing of several different drugs and/or delivery carriers.

Systems and methods for testing drugs and drug delivery systems

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West Lafayette, IN, United States of America

Bumsoo Han