Wisconsin Alumni Research Foundation

Erie Scientific Company

Gentel Bio Surfaces, Inc.

Bryce P Nelson

Robert M Corn

Robert M Goodman

James George Clements

Todd C Strother

David J Moore

Mark R Liles

John C Bart

Kendra Frederick

Chris Adrien

A multiwell plate device is provided having a frame, a substantially flat substrate and a multiwell structure supported by the substrate. The multiwell structure includes multiple bottomless wells formed therein. The substrate is supported by the frame and may be processed by an automated arrayer or instrument that is used to print or spot arrays in a pattern on a reaction surface of the substrate. Thereafter, the multiwell structure may be engaged with the substrate and the multiwell structure and substrate may be engaged with the frame in an upright orientation. For scanning or other analysis, the multiwell structure and substrate may be disengaged from the frame, inverted 180&#xb0;, and then reengaged with the frame in the inverted orientation.

Multiwell plate device

The present invention relates to novel methods for the analysis of molecular interactions. In particular, the present invention relates to compositions and methods for molecular interaction assays performed in solution and on solid surfaces. The present invention thus provides improves methods and compositions for the analysis of molecular interactions.

Molecular interaction assays on a solid surface

Disclosed is a method to detect unlabeled nucleic acids (DNA and/or RNA) in a taxa, species, and organelle-specific fashion using surface plasmon resonance (SPR) imaging. Taxa-specific, species-specific, or organelle-specific nucleic acids are affixed to an SPR-suitable substrate. A nucleic acid sample to be analyzed is then contacted with the SPR-substrate and the substrate analyzed to determine the presence or absence of specific hybridization between the nucleic acids bound to the substrate and the nucleic acids contained in the sample. The method does not require that either the bound nucleic acids nor the sample nucleic acids be labeled. The method can be used to identify the source of nucleic acids, their sequence, as well as to identify organisms and place them within a given taxonomic hierarchy.

Label-free detection of nucleic acids via surface plasmon resonance

Growing community of inventors

Madison, WI, United States of America

Bryce P Nelson