Institut National De La Sante Et De La Recherche Medicale

Centre National De La Recherche Scientifique

Université De Nantes

Bruno Pitard

Jean-Marie Lehn

Pierre Lehn

Charles Tellier

Jean-Pierre Vigneron

Corinne Miral

Emilie Letrou-Bonneval

The present invention relates to the use of at least one tetra functional non-ionic amphiphilic block copolymer as a vehicle for capped or uncapped mRNA for intracellular delivery for gene therapy.

Capped and uncapped RNA molecules and block copolymers for intracellular delivery of RNA

The present invention relates to a use of at least one glycosylated tetrafunctional amphiphilic block copolymer, as immune adjuvant.

Use of a glycosylated-modified tetrafunctional non-ionic amphiphilic block copolymer as immune adjuvant

A stabilized multimodular assembly for intracellular delivery comprising a complex of at least one cationic transfection agent and of at least one negatively charged macromolecule, wherein the complex has a theoretical charge ratio ranging from about 0 to about 4, and an efficient amount of at least one amphiphilic block co-polymer acting as a steric colloidal stabilizer with respect to the complex, the block co-polymer having hydrophilic and hydrophobic blocks wherein at least one hydrophilic block is conjugated with at least one targeting ligand.

Multimodular assembly useful for intracellular delivery

A pharmaceutical composition, including at least one nucleic acid, a Tyrode's medium having 140 mM NaC1, 6 mM KC1, 3 mM CaC1, 2 mM MgC1, 10 mM Hepes, pH 7.4, and 10 mM glucose; and a tetrafunctional copolymer of formula (I): where x and y represent, independently of one another, an integer of between 1 and 500, the tetrafunctional copolymer is in a form of a mineral salt wherein said tetrafunctional copolymer is in a cationic form. The compound of formula (I) has a molecular weight of between 1,000 and 25,000 g/mol and an EO/PO ratio of 0.8 to 1.2.

Pharmaceutical composition which improves in vivo gene transfer

The invention relates to a pharmaceutical composition which combines a tetrafunctional copolymer with a nucleic acid, said copolymer having formula I (namely a poloxamine), and preferably taking the form of one of the cationic mineral or organic salts thereof. The inventive composition can be used to improve in vivo gene transfer.

Growing community of inventors

Reze, France

Bruno Pitard