Amylum Belgium N.v.

Eurolysine

A E Staley Manufacturing Company

Bruno Jarry

Pierre Cami

Osamu Kurahashi

Anne Beyou

Robert J Jansen

Thomas Chattaway

Aharon Eyal

Asher Vitner

Didier Lecomte

Francedillaois Richaud

Ko&iuml;chi Takinami

Emmanuel Mailly

Francois Richaud

Jean Scott

Joelle More

Vitner Asher

Frank Van Lancker

Koichi Takinami

The invention provides a process for the production of aspartic acid poly-condensate from a carbohydrate comprising the steps of: fermenting a carbohydrate-containing medium by means of a fumaric acid-producing microorganism, whereby a fumarate-containing fermentation liquor is formed; forming a purified ammonium fumarate solution from the fumarate-containing fermentation liquor; enzymatically converting the purified ammonium fumarate into purified ammonium aspartate; heating an aqueous solution of an aspartate salt derived from the purified ammonium aspartate whereby water is removed, an aspartic acid condensate is formed and a second product is formed, which second product is basic and contains the cation of the aspartate salt; and removing and using the basic second product as a reagent in another step of the process.

Process for the production of aspartic acid condensate

Process for integration of a chosen gene on the chromosome of a bacterium obtained by the said process

Process for the production of crystalline aspartic acid

The present invention relates to a process for integration of a chosen gene or of a specific DNA molecule into the chromosome or episome of a bacterium by cloning the gene or DNA molecule into a defective transposon which is then integrated into the chromosomal or episomal DNA of the bacteria. The defective transposon is incapable of transposition autonomously but can be induced to transpose when properly complemented. The complementation to induce transposition can be limited so that the defective transposon produces a specific number of copies and thereafter is stable. The number of copies of the defective transposon produced during the period of transposition can be estimated by the level of expression of a marker gene contained within the defective transposon.

Process for integration of a chosen gene on the chromosome of a

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Paris, France

Bruno Jarry