Children's Hospital Research Center at Oakland

University of California

Juvenon, Inc.

Bruce N Ames

Hani Atamna

Pauline Gee

Qing Jiang

Dorothy M Maron

Frans A Kuypers

Tal Offer

Tory M Hagen

Emily Ho

Hani Alamna

Anti-inflammatory compositions include medicaments comprising predetermined amounts of a phytyl substituted chromanol and a prostaglandin Einhibitor, wherein: said medicament is in unit dosage form suitable for pharmaceutical administration; said phytyl substituted chromanol is a gamma-tocopherol, delta-tocopherol, gamma-tocotrienol or delta-tocotrienol; said PGEinhibitor is a non-steroidal anti-inflammatory drug or an omega-3 fatty acid, such as docosahexaenoic acid and eicosapentaenoic acid.

Tocopherol and tocotrienol aerosols

The invention is a method for analyzing immature reticulocytes for the presence of micronuclei. The method includes reticulocyte enrichment, fluorescent labeling, micronuclei staining, and analysis using single-laser flow cytometry. The invention also includes kits containing reagents to use in the method.

Flow cytometry based micronucleus assays and kits

Anti-obesity compositions include medicaments comprising predetermined amounts of a phytyl substituted chromanol and an obesity-promoting drug, wherein: said medicament is in unit dosage form suitable for pharmaceutical administration; said phytyl substituted chromanol is a gamma-tocopherol, delta-tocopherol, alpha-tocotrienol, gamma-tocotrienol or delta-tocotrienol; said obesity-promoting drug is a corticosteroid or an anti-diabetes drug such as a hypoglycemic drug, starch blocker, glucose production blocker or insulin sensitizer.

Tocopherol and tocotrienol anti-obesity medicaments

A&#x3b2;-heme peroxidase activity is selectively measured and inhibited, packaged as a pharmaceutical composition with heme biosynthetic cofactors or as an industrial enzyme, and used to oxidize target substrates.

A&#x3b2;-heme peroxidase

A method of stabilizing R-&#x3b1;-lipoic acid includes combining R-&#x3b1;-lipoic acid with nicotinamide in a weight ratio between about 10:4 to about 10:8. A composition for treating oxidative stress includes R-&#x3b1;-lipoic acid and nicotinamide in a weight ratio of between about 10:4 and about 10:8.

Stability of lipoic acid

Pharmaceutical nitrone comprise condensates of an N-hydroxylamine and a physiological aldehyde, providing improved delivery and absorption, enhanced stability and reduced toxicity. Preferred physiological aldehydes are subject to endogenous cellular uptake transport, and include pyridoxal, pyridoxal phosphate, and heme-A. Essentially any physiologically compatible and pharmaceutically active hydroxylamine moiety may be incorporated, such as hydroxylamine moieties of prior pharmaceutical nitrones, and known pharmaceutically active hydroxylamines.

Pharmaceutical nitrones

The invention provides pharmaceutical compositions comprising primary N-hydroxylamines and related therapeutic, prophylactic, diagnostic and screening methods. The pharmaceutical compositions generally comprise a pharmaceutical composition comprising an orally administrable effective unit solid dosage of a primary N-hydroxylamine or a pharmaceutically acceptable salt thereof and substantially free of a nitrone corresponding to the hydroxylamine.

Primary N-hydroxylamines

The metabolic rate of aged cells of a mammalian host is enhanced without a concomitant increase in metabolic production of reactive oxygen species by orally administering to the host an effective dosage of a carnitine, such as acetyl-L-carnitine, and a mitochondrially active antioxidant, such as lipoic acid.

Dietary composition for enhancing metabolism and alleviating oxidative

Six sets of Salmonella typhimurium strains are provided, all strains having low spontaneous mutation rates, and each set being sensitive to and diagnostic of one of the six possible base substitutions in DNA. Also provided are methods for making and using these S. typhimurium strains to detect, and establish the type of specific base substitution mutation induced by, mutagenic agents.

Growing community of inventors

Berkeley, CA, United States of America

Bruce N Ames