Ut-battelle, Inc.

Other

Purdue Research Foundation

University of Tennessee Research Foundation

Critical Point Technologies, Inc.

National Water Research Institute

Bruce Michael Applegate

Gary S Sayler

Michael Leon Simpson

Steven Ripp

Michael J Paulus

David E Nivens

Ernest Rowland Blatchley, Iii

Mark Thomas Morgan

James T Fleming

Aparna Kothapalli

Ferhan Ozadali

Eric Gentil Mbonimpa

Bryan Vadheim

Dharmendra K Mishra

Fernanda San Martin-Gonzalez

Lynda Louise Perry

A rapid cooling system for processing food is disclosed which includes a heat exchanger adapted to receive a first coolant (Coolant-I) at a first temperature and eject Coolant-I at a second temperature, a cooling chamber disposed within the heat exchanger in thermal communication with the heat exchanger, the cooling chamber includes a first inlet adapted to receive a product at an elevated temperature (T), a second inlet adapted to receive a second coolant (Coolant-II) at a low temperature (T), and an outlet adapted to release a combination of the product and Coolant-II at a low temperature (T) and pressure (P), wherein cooling of the product from Tto Tdoes not cause a phase change in the product.

Cooling apparatus and method of using the same

Potable drinking water is a scarce resource in many parts of developing countries, especially rural areas. Due to limited financial means of these countries, low cost point-of-use systems are thought to be appropriate technology to treat water. Systems using solar ultraviolet (UV) radiation could be successful since many vulnerable countries are located where solar radiation is intense and abundant throughout the year. The goal of this disclosure is to develop a simple and low cost point-of-use solar UV reactor to disinfect water. In this disclosure wavelength-dependent microbial dose-response behavior was investigated using surrogates to pathogenic microbes. A solar radiation prediction method based on the SMARTS model was used to predict solar UV intensity as function of geographic location and time. A numerical modeling procedure using the discrete ordinate (DO) model and CFD software (FLUENT) was used to simulate UV dose (distribution) delivery to microorganisms. Then, the dose distribution was combined with the dose response behavior using a segregated flow model to predict microbial inactivation by the reactor. A prototype was produced and tested to validate the numerical modeling procedure. The inactivation results from the prototype were in agreement with numerical inactivation prediction. The modeling procedure permits parameters such as reactor dimensions and material properties to be varied to meet a treatment goal.

Continuous-flow solar ultraviolet disinfection system for drinking water

Novel reporter bacteriophages are provided. Provided are compositions and methods that allow bacteriophages that are used for specific detection or killing of0157:H7 to be propagated in nonpathogenic, thereby eliminating the safety and security risks of propagation in0157:H7. Provided are compositions and methods for attaching active bacteriophages to the surface of a polymer in order to kill target bacteria with which the phage comes into contact. Provided are modified bacteriophages immobilized to a surface, which capture0157:H7 and cause the captured cells to emit light or fluorescence, allowing detection of the bacteria in a sample.

Methods for generation of reporter phages and immobilization of active bacteriophages on a polymer surface

An integrated microluminometer includes an integrated circuit chip having at least one n-well/p-substrate junction photodetector for converting light received into a photocurrent, and a detector on the chip for processing the photocurrent. A distributed electrode configuration including a plurality of spaced apart electrodes disposed on an active region of the photodetector is preferably used to raise efficiency.

Microluminometer chip and method to measure bioluminescence

Monolithic bioelectronic devices for the detection of ammonia includes a microorganism that metabolizes ammonia and which harbors a lux gene fused with a heterologous promoter gene stably incorporated into the chromosome of the microorganism and an Optical Application Specific Integrated Circuit (OASIC). The microorganism is generally a bacterium.

Bioluminescent bioreporter integrated circuit devices and methods for detecting ammonia

Bioelectronic devices for the detection of estrogen include a collection of eukaryotic cells which harbor a recombinant lux gene from a high temperature microorganism wherein the gene is operably linked with a heterologous promoter gene. A detectable light-emitting lux gene product is expressed in the presence of the estrogen and detected by the device.

Bioluminescent bioreporter integrated circuit devices and methods for detecting estrogen

A method for cell-based combinatorial logic includes the steps of providing at least one genetically engineered cell, the genetically engineered cell having at least one transcriptional unit. The transcriptional unit includes a gene and a promoter, wherein application of a stimulus to the promoter results in the expression of a gene product. An energetic or chemical stimulus is applied to activate the promoter, wherein the detection of an output signal corresponds to the presence of a gene product. The cell can include a plurality of transcriptional units configured to form logic gates. The logic gates of a plurality of cells can be operably interconnected by release of output signals, such as chemical stimuli.

Methods for cell-based combinatorial logic

Disclosed are monolithic bioelectronic devices comprising a bioreporter and an OASIC. These bioluminescent bioreporter integrated circuit are useful in detecting substances such as pollutants, explosives, and heavy-metals residing in inhospitable areas such as groundwater, industrial process vessels, and battlefields. Also disclosed are methods and apparatus for detection of particular analytes, including ammonia and estrogen compounds.

Bioluminescent bioreporter integrated circuit detection methods

Disclosed are bioluminescent bioreporter integrated circuit devices that detect selected analytes in fluids when implanted in the body of an animal. The device comprises a bioreporter that has been genetically engineered to contain a nucleic acid segment that comprises a cis-activating response element that is responsive to the selected substance operably linked to a gene encoding a bioluminescent reporter polypeptide. In preferred embodiments, the target analyte is glucose, glucagons, or insulin. Exposure of the bioreporter to the target substance causes the response element to up-regulate the nucleic acid sequence encoding the reporter polypeptide to produce a luminescent response that is detected and quantitated. In illustrative embodiments, the bioreporter device is encapsulated on an integrated circuit that is capable of detecting the emitted light, processing the resultant signal, and then remotely reporting the results. Also disclosed are controlled drug delivery systems capable of being directly or indirectly controlled by the detection device that provide drugs such as insulin to the animal in reponse to the amount of target analyte present in the body fluids.

In vivo biosensor apparatus and method of use

Disclosed are methods and devices for detection of bacteria based on recognition and infection of one or more selected strains of bacteria with bacteriophage genetically modified to cause production of an inducer molecule in the bacterium following phage infection. The inducer molecule is released from the infected bacterium and is detected by genetically modified bacterial bioreporter cells designed to emit bioluminescence upon stimulation by the inducer. Autoamplification of the bioluminescent signal permits detection of low levels of bacteria without sample enrichment. Also disclosed are methods of detection for select bacteria, and kits for detection of select bacteria based on the described technology.

Bioluminescent biosensor device

A method for detecting the presence of a microorganism in an environmental sample involves contacting the sample with a supercritical fluid to isolate nucleic acid from the microorganism, then detecting the presence of a particular sequence within the isolated nucleic acid. The nucleic acid may optionally be subjected to further purification.

Growing community of inventors

West Lafayette, IN, United States of America

Bruce Michael Applegate