City of Hope

Systec Corporation

Bruce E Kaplan

Steven S Smith

John J Rossi

Carl W Sims

Piotr Marek Swiderski

Keiichi Itakura

Richard J Zelinka

Pairoj Chang

A reverse transcriptase assay is described. A modular nanostructure comprising relatively small and relatively long RNA sequences is utilized in the assay.

Reverse transcriptase detection and inhibition

A disposable cartridge for storage and delivery of reagents for synthesizing and labeling of synthetic oligonucleotides comprises an annular casing, an enclosed area defined by the interior surface of the casing and a non-swellable matrix which is inert to organic solvents is positioned within the enclosed area. Adsorbed onto the surface of the matrix is a reagent to be coupled to an immobilized oligonucleotide precursor. The cartridges can be packaged in such a way as to insure the long-term stability of the reagents contained therein. The cartridge is readily insertable into the reagent delivery line of an automated synthesizer of oligonucleotides and also is useful for the production of labeled oligonucleotides by manual coupling. The cartridges of the invention are disposable after a single use. Substantial economies in the synthesis of labeled synthetic oligonucleotides are achieved by use of the cartridge.

Disposable reagent storage and delivery cartridge

Molecules having an RNA block linked to a shorter DNA block is described. The RNA and DNA blocks are complementary to accommodate formation of fold-back molecules having a 3' hydroxyl on the DNA block and an overhanging RNA strand at the end of a short DNA-RNA hybrid.

RNA-DNA hybrid molecules of nucleic acid

Mechanism based inhibitors of DNA methyltransferase

This invention provides chimeric DNA/RNA catalytic molecules useful to cleave RNA sequences.

Chimeric DNA-RNA catalytic sequences

A microprocessor controlled, general purpose gene synthesizer for programmably synthesizing selected nucleotide sequences. Depending upon a programmed chemistry, metered volumes of selected bases and reagent/solvents are sequentially metered into the bottom of a reaction cell containing solid-supported nucleotides, where desired linkages are achieved in an agitated suspension. Self-refilling syringe metering and electrically initiated, pneumatically operated valving insures economical, non-contaminated synthesis. Preprogrammed, selectable chemistries and operator programmed sequences are operatively coupled under microprocessor control to ensure a general purpose synthesizer.

Growing community of inventors

Claremont, CA, United States of America

Bruce E Kaplan