University of California

The J. David Gladstone Institutes

Bio-rad Laboratories, Inc.

The J. David Gladstone Institutes, a Testamentary Trust Established Under the Will of J. David Gladstone

Other

Bruce Conklin

Kevin Edward Healy

Samantha Cooper

George Karlin-Neumann

Josh Shinoff

Jennifer Berman

Nathaniel Huebsch

Yuichiro Miyaoka

Peter Loskill

J&uuml;rgen Wess

Evi Kostenis

Luke Judge

Josh Schinoff

The invention relates to devices, methods, kits, and compositions for in vitro generation of three-dimensional micro-tissues that are accurate models of heart, skeletal muscle, neuronal, and other tissues.

Reversible stencils for fabricating micro-tissues

Methods, compositions, and kits are provided for quantification of genome editing.

Detection of genome editing

The present invention provides assays and compositions to identify the risk of toxicity in a patient population with genotypic variations in specific proteins and/or protein complexes within the patient population.

Method of determining cellular protein homeostasis

The invention provides a method for selectively activating a target cell, where the target cell expresses a receptor activated superiorly by a synthetic ligand (RASSL) having decreased binding affinity for a selected natural ligand and normal or near normal binding affinity for a synthetic small molecule agonist. Thus, RASSL-mediated activation of target cells does not occur to a significant extent in the presence of natural G protein-coupled receptor ligand, but is significantly stimulated upon exposure to a synthetic small molecule. RASSL-expressing target cells are selectively activated by exposing of the cells to an appropriate synthetic small molecule, which in turn binds the RASSL, resulting in G protein activation and triggering of a specific cellular response associated with G protein activation (e.g., cellular proliferation or cellular secretion).

Selective target cell activation by expression of a G protein-coupled receptor activated superiorly by synthetic ligand

The present invention provides methods and compositions that can be used to identify modulators of G-protein-coupled receptors.

Methods and compositions for identifying modulators of G-protein-coupled receptors

Growing community of inventors

San Francisco, CA, United States of America

Bruce Conklin