Hoffmann-la Roche Inc.

Bruce Charles Farrenkopf

Richard A Kaufman

Magdalena U Gomez

A method for determining total digoxin levels in a biological fluid sample containing digoxin-protein complex which method comprises disrupting the digoxin-protein complex by treating said sample solution with an effective amount of a dissociation agent selected from one or more members of the group consisting of a C.sub.3 -C.sub.26 saturated or unsaturated fatty acid, a C.sub.2-7 lower alkanol, quinidine and .alpha.-tocopherol so as to release the bound digoxin from serum protein, passing the treated sample solution through a centrifugal ultrafilter so as to selectively pass digoxin containing sample solution through said filter and retaining said serum proteins and determining the digoxin contents in said sample solution in a fluorescence polarization assay. A kit for carrying out the method is provided.

Method and kit for determining total digoxin levels involving agent to

Angiotensin I and iodinated angiotensin I solutions are stabilized against enzymatic degradation by the addition of phenylmethylsulfonyl fluoride (PMSK). Such solutions are used as standards and reagents in immunoassay kits for determinatin of plasma renin levels. Stabilized solutions prepared in accordance with this disclosure can be shipped at ambient temperature which represents a substantial advantage in convenience over previously available solutions of angiotensin I and radioiodinated angiotensin I.

Growing community of inventors

Clifton, NJ, United States of America

Bruce Charles Farrenkopf